Liu Xueqing, Liu Hongrun, Zhang Yushi, Liu Churong, Liu Yanan, Li Zhaohu, Zhang Mingcai
State Key Laboratory of Plant Environmental Resilience, Ministry of Education, Key Laboratory of Farming System, Ministry of Agriculture of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
State Key Laboratory of Plant Environmental Resilience, Ministry of Education, Key Laboratory of Farming System, Ministry of Agriculture of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
J Environ Manage. 2023 Aug 1;339:117927. doi: 10.1016/j.jenvman.2023.117927. Epub 2023 Apr 17.
Straw retention (SR) and organic fertilizer (OF) application contribute to improve soil quality, but it is unclear how the soil microbial assemblage under organic amendments mediate soil biochemical metabolism pathways to perform it. This study collected soil samples from wheat field under different application of fertilizer (chemical fertilizer, as control; SR, and OF) in North China Plain, and systematically investigated the interlinkages among microbe assemblages, metabolites, and physicochemical properties. Results showed that the soil organic carbon (SOC) and permanganate oxidizable organic carbon (LOC) in soil samples followed the trend as OF > SR > control, and the activity of C-acquiring enzymes presented significantly positive correlation with SOC and LOC. In organic amendments, bacteria and fungi community were respectively dominated by deterministic and stochastic processes, while OF exerted more selective pressure on soil microbe. Compared with SR, OF had greater potential to boost the microbial community robustness through increasing the natural connectivity and stimulating fungal taxa activities in inter-kingdom microbial networks. Altogether 67 soil metabolites were significantly affected by organic amendments, most of them belonged to benzenoids (Ben), lipids and lipid-like molecules (LL), and organic acids and derivatives (OA). These metabolites were mainly derived from lipid and amino acid metabolism pathways. A list of keystone genera such as stachybotrys and phytohabitans were identified as important to soil metabolites, SOC, and C-acquiring enzyme activity. Structural equation modeling showed that soil quality properties were closely associated with LL, OA, and PP drove by microbial community assembly and keystone genera. Overall, these findings suggested that straw and organic fertilizer might drive keystone genera dominated by determinism to mediate soil lipid and amino acid metabolism for improving soil quality, which provided new insights into understanding the microbial-mediated biological process in amending soil quality.
秸秆还田（SR）和施用有机肥（OF）有助于改善土壤质量，但尚不清楚有机改良措施下的土壤微生物群落如何介导土壤生化代谢途径来实现这一目标。本研究采集了华北平原不同施肥处理（化肥，作为对照；秸秆还田和施用有机肥）的麦田土壤样本，系统研究了微生物群落、代谢产物和理化性质之间的相互联系。结果表明，土壤样本中的土壤有机碳（SOC）和高锰酸盐氧化有机碳（LOC）呈现出OF＞SR＞对照的趋势，且碳获取酶的活性与SOC和LOC呈显著正相关。在有机改良措施中，细菌和真菌群落分别由确定性和随机性过程主导，而有机肥对土壤微生物施加了更大的选择压力。与秸秆还田相比，有机肥通过增加自然连通性和刺激跨王国微生物网络中的真菌类群活动，具有更大的潜力来提高微生物群落的稳健性。共有67种土壤代谢产物受到有机改良措施的显著影响，其中大多数属于苯类化合物（Ben）、脂质和类脂分子（LL）以及有机酸及其衍生物（OA）。这些代谢产物主要来自脂质和氨基酸代谢途径。鉴定出了一系列关键属，如葡萄穗霉属和植物栖息菌属，它们对土壤代谢产物、SOC和碳获取酶活性很重要。结构方程模型表明，土壤质量属性与微生物群落组装和关键属驱动的LL、OA和PP密切相关。总体而言，这些发现表明，秸秆和有机肥可能驱动以确定性为主导的关键属来介导土壤脂质和氨基酸代谢以改善土壤质量，这为理解改良土壤质量过程中微生物介导的生物学过程提供了新的见解。
