State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen University, Xiamen, 361102, People's Republic of China.
Department of Burn, Beijing Jishuitan Hospital, Beijing, 100035, People's Republic of China.
Sci Rep. 2023 Apr 19;13(1):6399. doi: 10.1038/s41598-023-33372-z.
Diabetes may leave patients more prone to skin problems, and minor skin conditions can more easily turn into serious damage to the extracellular matrix, which further impairs the skin's mechanical properties and delays wound healing. Therefore, the aim of the work is to develop extracellular matrix substitution to remodel the mechanical properties of diabetic cutaneous wound and thus accelerate diabetic wound healing. A green fabrication approach was used to prepare radiation crosslinked bilayer collagen scaffold from collagen dispersion. The morphological, mechanical and swelling characteristics of radiation crosslinked bilayer collagen scaffold were assessed to be suitable for cutaneous wound remodeling. The feasibility of radiation crosslinked bilayer collagen scaffold was performed on full-skin defect of streptozotocin-induced diabetic rats. The tissue specimens were harvested after 7, 14, and 21 days. Histopathological analysis showed that radiation crosslinked bilayer collagen scaffold has beneficial effects on inducing skin regeneration and remodeling in diabetic rats. In addition, immunohistochemical staining further revealed that the radiation crosslinked bilayer collagen scaffold could not only significantly accelerate the diabetic wound healing, but also promote angiogenesis factor (CD31) production. Vascularization was observed as early as day 7. The work expands the therapeutic ideas for cutaneous wound healing in diabetes.
糖尿病可能使患者更容易出现皮肤问题，而轻微的皮肤状况更容易导致细胞外基质的严重损伤，这进一步损害了皮肤的机械性能并延迟了伤口愈合。因此，本工作的目的是开发细胞外基质替代物，重塑糖尿病性皮肤伤口的机械性能，从而加速糖尿病性伤口愈合。采用绿色制造方法，从胶原分散液中制备了辐射交联双层胶原支架。评估了辐射交联双层胶原支架的形态、机械和溶胀特性，以适合皮肤伤口重塑。在链脲佐菌素诱导的糖尿病大鼠的全层皮肤缺损上进行了辐射交联双层胶原支架的可行性研究。在第 7、14 和 21 天采集组织标本。组织学分析表明，辐射交联双层胶原支架对诱导糖尿病大鼠皮肤再生和重塑具有有益作用。此外，免疫组织化学染色进一步表明，辐射交联双层胶原支架不仅能显著加速糖尿病创面愈合，还能促进血管生成因子（CD31）的产生。早在第 7 天就观察到了血管化。这项工作扩展了糖尿病皮肤伤口愈合的治疗思路。
