Nazarov Dmitriy A, Denisenko Georgy M, Budylin Gleb S, Kozlova Elizaveta A, Lipina Marina M, Lazarev Vladimir A, Shirshin Evgeny A, Tarabrin Mikhail K
Bauman Moscow State Technical University, Moscow, 105005, Russia.
Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia.
Biomed Opt Express. 2023 Mar 10;14(4):1509-1521. doi: 10.1364/BOE.483135. eCollection 2023 Apr 1.
Studies of the optical properties of biological tissues in the infrared range have demonstrated significant potential for diagnostic tasks. One of the insufficiently explored ranges for diagnostic problems at the moment is the fourth transparency window, or short wavelength infrared region II (SWIR II). A Cr:ZnSe laser with tuning capability in the range from 2.1 to 2.4 µm was developed to explore the possibilities in this region. The capability of diffuse reflectance spectroscopy to analyze water and collagen content in biosamples was investigated using the optical gelatin phantoms and the cartilage tissue samples during their drying process. It was demonstrated that decomposition components of the optical density spectra correlated with the partial content of the collagen and water in the samples. The present study indicates the possibility of using this spectral range for the development of diagnostic methods, in particular, for observation of the changes in the content of cartilage tissue components in degenerative diseases such as osteoarthritis.
对生物组织在红外波段光学特性的研究已证明其在诊断任务方面具有巨大潜力。目前，诊断问题中一个尚未得到充分探索的波段是第四透明窗口，即短波红外区域II（SWIR II）。为探索该区域的可能性，研制了一种调谐范围在2.1至2.4微米的Cr:ZnSe激光器。利用光学明胶模型和软骨组织样本在干燥过程中的情况，研究了漫反射光谱分析生物样本中水和胶原蛋白含量的能力。结果表明，光密度光谱的分解成分与样本中胶原蛋白和水的部分含量相关。本研究表明，利用该光谱范围开发诊断方法是可行的，特别是用于观察诸如骨关节炎等退行性疾病中软骨组织成分含量的变化。
