Sohmen Maximilian, Muñoz-Bolaños Juan D, Rajaeipour Pouya, Ritsch-Marte Monika, Ataman Çağlar, Jesacher Alexander
Institute for Biomedical Physics, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.
Phaseform GmbH, Georges-Köhler-Allee 102, 79110 Freiburg, Germany.
Biomed Opt Express. 2023 Mar 20;14(4):1562-1578. doi: 10.1364/BOE.481453. eCollection 2023 Apr 1.
Adaptive optics, in combination with multi-photon techniques, is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators that are reflective, diffractive or both. This, however, can pose a severe limitation for applications. Here, we present a fast and robust sensorless adaptive optics scheme adapted for transmissive wavefront modulators. We study our scheme in numerical simulations and in experiments with a novel, optofluidic wavefront shaping device that is transmissive, refractive, polarisation-independent, and broadband. We demonstrate scatter correction of two-photon-excited fluorescence images of microbeads as well as brain cells and benchmark our device against a liquid-crystal spatial light modulator. Our method and technology could open new routes for adaptive optics in scenarios where previously, the restriction to reflective and diffractive devices may have staggered innovation and progress.
自适应光学与多光子技术相结合，是一种深入观察样本内部的强大成像方法。值得注意的是，如今几乎所有的自适应光学方案都依赖于反射式、衍射式或兼具两者的波前调制器。然而，这可能会给应用带来严重限制。在此，我们提出一种适用于透射式波前调制器的快速且稳健的无传感器自适应光学方案。我们通过数值模拟以及使用一种新型的光流体波前整形装置进行实验来研究我们的方案，该装置具有透射性、折射性、与偏振无关且宽带的特性。我们展示了对微珠以及脑细胞的双光子激发荧光图像的散射校正，并将我们的装置与液晶空间光调制器进行了对比测试。我们的方法和技术可以为自适应光学开辟新的途径，在以前，对反射式和衍射式装置的限制可能阻碍了创新和进展的情况下。
