Papadopoulos Ioannis N, Jouhanneau Jean-Sebastien, Takahashi Naoya, Kaplan David, Larkum Matthew, Poulet James, Judkewitz Benjamin
Charité - Universitätsmedizin Berlin, Einstein Center for Neurosciences, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany.
Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13092 Berlin, Germany.
Light Sci Appl. 2020 Jun 30;9:110. doi: 10.1038/s41377-020-00348-x. eCollection 2020.
Optical microscopy is an indispensable tool in biomedical sciences, but its reach in deep tissues is limited due to aberrations and scattering. This problem can be overcome by wavefront-shaping techniques, albeit at limited fields of view (FOVs). Inspired by astronomical imaging, conjugate wavefront shaping can lead to an increased field of view in microscopy, but this correction is limited to a set depth and cannot be dynamically adapted. Here, we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing (F-SHARP). We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes. We demonstrate the power of the method by imaging with high resolution over extended FOV (>80 µm) deeper than 400 μm inside a mouse brain through a thinned skull.
光学显微镜是生物医学科学中不可或缺的工具，但由于像差和散射，其在深部组织中的成像深度有限。尽管视场（FOV）有限，但通过波前整形技术可以克服这个问题。受天文成像的启发，共轭波前整形可以增加显微镜的视场，但这种校正仅限于特定深度，且无法动态调整。在这里，我们提出了一种基于聚焦扫描全息像差探测（F-SHARP）的共轭波前整形方案。我们将其与一种紧凑的实现方式相结合，这种方式可以很容易地应用于各种商用和自制的双光子显微镜。我们通过在小鼠脑内透过变薄的颅骨，在超过80μm的扩展视场且深度超过400μm的范围内进行高分辨率成像，展示了该方法的强大功能。
