一种用于微量测定高碘酸盐的分光光度法。
A spectrophotometric method for the microdetermination of periodate.
作者信息
Fields R, Dixon H B
出版信息
Biochem J. 1968 Aug;108(5):883-7. doi: 10.1042/bj1080883.
Abstract
- A method is described for measuring the concentration of periodate over the range 0.2-20mum by adding 1,2-di-(p-dimethylaminophenyl)ethane-1,2-diol to a sample solution. Periodate cleaves this compound to from two molecules of p-dimethylaminobenzaldehyde, the extinction of which is then read at 352mmu. 2. The method has been used to follow the course of periodate oxidations of serine methyl ester, ribonuclease A and ribonuclease S-protein. Addition of the reagent stops further periodate reaction by reducing the remaining periodate to iodate. 3. The presence of protein does not interfere with the assay.
摘要
- 描述了一种通过向样品溶液中加入1,2 - 二 -（对 - 二甲氨基苯基）乙烷 - 1,2 - 二醇来测量0.2 - 20μm范围内高碘酸盐浓度的方法。高碘酸盐将该化合物裂解形成两分子对 - 二甲氨基苯甲醛，然后在352nm处读取其吸光度。2. 该方法已用于跟踪丝氨酸甲酯、核糖核酸酶A和核糖核酸酶S - 蛋白的高碘酸盐氧化过程。加入试剂通过将剩余的高碘酸盐还原为碘酸盐来停止进一步的高碘酸盐反应。3. 蛋白质的存在不干扰测定。
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