大肠杆菌K-12染色体复制起点的核苷酸序列。
Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
作者信息
Meijer M, Beck E, Hansen F G, Bergmans H E, Messer W, von Meyenburg K, Schaller H
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4. doi: 10.1073/pnas.76.2.580.
Abstract
The origin of replication, oriC, of the Escherichia coli chromosome was mapped within a DNA segment of 422 base pairs. The nucleotide sequence of this segment was determined. The source of DNA for the sequence analysis was a minichromosome constructed in vivo, consisting exclusively of chromosomal DNA and a minichromosome constructed by cloning in vitro. The nucleotide sequence of the replication origin is characterized by a high degree of repetitiveness due to both inverted and direct repeats. Sequence homologies were found between portions of the replication origins of E. coli and phages lambda and G4. This suggests similarities in some steps in the initiation of replication of the different replicons.
摘要
大肠杆菌染色体的复制起点oriC被定位在一段422个碱基对的DNA片段内。测定了该片段的核苷酸序列。用于序列分析的DNA来源是在体内构建的微型染色体，它仅由染色体DNA组成，以及通过体外克隆构建的微型染色体。复制起点的核苷酸序列由于反向重复和正向重复而具有高度重复性。在大肠杆菌与噬菌体λ和G4的复制起点部分之间发现了序列同源性。这表明不同复制子在复制起始的某些步骤中存在相似性。
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Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4. doi: 10.1073/pnas.76.2.580.
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