噬菌体φX174 RF DNA复制起点的核苷酸序列。
Nucleotide sequence of the origin of replication in bacteriophage phiX174 RF DNA.
作者信息
Langeveld S A, van Mansfeld A D, Baas P D, Jansz H S, van Arkel G A, Weisbeek P J
出版信息
Nature. 1978 Feb 2;271(5644):417-20. doi: 10.1038/271417a0.
DOI:10.1038/271417a0
PMID:628424
Abstract
The gene A protein of bacteriophage phiX174 has been used in vitro to convert phiX RFI DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3' end of the nick has been determined as -- T G C T C C C C C A A C T T Goh. This sequence gives the exact position of the origin of phiX RF DNA replication.
摘要
噬菌体φX174的基因A蛋白已在体外用于通过切割病毒链将φX RFI DNA转化为松弛的RFII形式。切口3'端的核苷酸序列已确定为——T G C T C C C C C A A C T T Goh。该序列给出了φX RF DNA复制起点的确切位置。
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