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从利什曼原虫寄生虫中分离出的小细胞外囊泡的DNA含量鉴定与分析。

Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites.

作者信息

Ibarra-Meneses Ana Victoria, Amin Atia, Dong George, Olivier Martin, Langlais David, Fernandez-Prada Christopher

机构信息

Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, 626 CIMIA Sicotte Street, Saint-Hyacinthe, QC J2S 2M2, Canada; The Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, Canada.

Department of Human Genetics, Dahdaleh Institute for Genomic Medicine, Montreal, QC H3A 0G1, Canada.

出版信息

STAR Protoc. 2023 Apr 21;4(2):102248. doi: 10.1016/j.xpro.2023.102248.

DOI:10.1016/j.xpro.2023.102248
PMID:37087735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10160803/
Abstract

Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022)..

摘要

在此，我们聚焦于利什曼原虫细胞外囊泡（EVs）及其DNA含量，详细介绍了分离这些纳米颗粒及其后续基因组特征分析的方案。我们描述了一种用于获取、储存和分析源自培养寄生虫的EVs的强大且全面的方法。我们详细介绍了一种用户友好的生物信息学流程，用于序列分析以及拷贝数变异（CNV）分析和倍性变化的可视化。有关此方案的使用和执行的完整详细信息，请参考杜阿纳等人（2022年）的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/5d8e7b9ebd43/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/458e156ce8ac/fx1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/6b4394c17a9d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/ac4ce4d42495/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/65976668cdc7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/dc8585ad0128/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/0ff0c71a3364/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/508c8b40bb3f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/fa8edac80384/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/4184d352871f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/983c82d12c8f/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/45494919a540/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/6fc2accdd80a/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/5d8e7b9ebd43/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/458e156ce8ac/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/d23c602df3c1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/6b4394c17a9d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/ac4ce4d42495/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/65976668cdc7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/dc8585ad0128/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/0ff0c71a3364/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/508c8b40bb3f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/fa8edac80384/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/4184d352871f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/983c82d12c8f/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/45494919a540/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/6fc2accdd80a/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3fc/10160803/5d8e7b9ebd43/gr13.jpg

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