Khadir Fatemeh, Rahimi Zohreh, Ghanbarpour Azita, Vaisi-Raygani Asad
Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Mol Cell Med. 2022;11(2):127-136. doi: 10.22088/IJMCM.BUMS.11.2.127. Epub 2022 Nov 27.
Preeclampsia as a multifactor hypertensive disorder of pregnancy is associated with enhanced placental oxidative stress. The Keap1-Nrf2 pathway protects cells against oxidative stress. We examined the possible association between the variants in relation to oxidative stress parameters with the risk of preeclampsia. We studied 150 preeclampsia women and 150 women with a normal pregnancy to find the frequency of rs6721961 genotypes using the PCR-RFLP method. Also, an association between the genotypes with the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) was analyzed. Significantly lower TAC and higher MDA levels were found in preeclampsia patients compared to controls (P<0.0001). For the first time, we report an association between the rs6721961 polymorphism and preeclampsia risk. The present study indicated that the GT genotype and the T allele of the rs6721961 increased the risk of preeclampsia by 2.81 and 2.39 times, respectively. Also, the TT genotype was associated with a 3.9-fold increased risk of early-onset preeclampsia. We detected a positive association between the levels of body mass index, MDA, and the polymorphism with the risk of preeclampsia and a negative correlation between the level of TAC with the preeclampsia risk. Also, an association between the rs6721961 TT genotype with higher serum MDA levels was found. Our study suggests oxidative stress is involved in the pathogenesis of preeclampsia and the rs6721961 polymorphism through alteration in the levels of oxidative stress parameters might increase the risk of preeclampsia and early-onset preeclampsia.
子痫前期作为一种妊娠多因素高血压疾病，与胎盘氧化应激增强有关。Keap1-Nrf2通路可保护细胞免受氧化应激。我们研究了与氧化应激参数相关的变异与子痫前期风险之间的可能关联。我们研究了150名单纯性高血压孕妇和150名正常妊娠妇女，采用PCR-RFLP方法确定rs6721961基因型的频率。此外，分析了这些基因型与丙二醛（MDA）水平和总抗氧化能力（TAC）之间的关联。与对照组相比，子痫前期患者的TAC显著降低，MDA水平显著升高（P<0.0001）。我们首次报道了rs6721961多态性与子痫前期风险之间的关联。本研究表明，rs6721961的GT基因型和T等位基因使子痫前期风险分别增加2.81倍和2.39倍。此外，TT基因型与早发型子痫前期风险增加3.9倍有关。我们发现体重指数、MDA水平与该多态性与子痫前期风险呈正相关，而TAC水平与子痫前期风险呈负相关。此外，还发现rs6721961 TT基因型与较高的血清MDA水平有关。我们的研究表明，氧化应激通过氧化应激参数水平的改变参与子痫前期的发病机制，而rs6721961多态性可能增加子痫前期和早发型子痫前期的风险。
