Department of Burn and Plastic Surgery, Institute of Translational Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210002, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Adv Mater. 2023 Jul;35(28):e2301064. doi: 10.1002/adma.202301064. Epub 2023 May 26.
Wound dressing patches based on stem cells have demonstrated promising potential in the treatment of diabetic wounds, while their capabilities in the maintenance of cell stemness, effective exchange of cellular substance, and precise targeting of the stem cells remain to be promoted. Here, a novel stem cell spheroid loaded microneedle (MN@SPs) patch is presented using microfluidic templating technology. By leveraging the precise fluid manipulation capabilities of the microfluidic template, stem cell spheroids (SPs) with uniform size can be generated in situ. The resulting SPs exhibit advanced viability and cell functions, as evidenced by the overexpressed genes related to extracellular matrix organization and angiogenesis. By loading these SPs into the microneedles (MNs), the platform enables the precise delivery and exchange of multiple active substances, which contribute to advanced neovascularization, collagen deposition, and tissue reconstruction of diabetic wounds. Overall, this microfluidic-engineered stem cell therapy platform demonstrates significant promise in promoting wound healing.
基于干细胞的创伤敷料贴片在治疗糖尿病创面方面显示出了巨大的潜力，然而，其在维持细胞干性、有效细胞物质交换和精准靶向干细胞方面的能力仍有待提高。在这里，我们使用微流控模板技术提出了一种新型的负载干细胞球体的微针贴片（MN@SPs）。通过利用微流控模板的精确流体操控能力，可以在原位生成具有均匀尺寸的干细胞球体（SPs）。结果表明，这些 SPs 具有较高的活力和细胞功能，与细胞外基质组织和血管生成相关的基因表达上调。通过将这些 SPs 装载到微针（MNs）中，该平台可以实现多种活性物质的精确传递和交换，从而促进糖尿病创面的新生血管形成、胶原沉积和组织重建。总的来说，这种基于微流控技术的干细胞治疗平台在促进创面愈合方面具有巨大的应用潜力。
