Park Juyoung, Lee Handule, Choi Dal-Woong, Park Kwangsik
College of Pharmacy, Dongduk Women's University, Seoul 02748, Republic of Korea.
Department of Public Health Sciences, Transdisciplinary Major in Learning Health Systems, Graduate School, Korea University, Seoul 02481, Republic of Korea.
Environ Anal Health Toxicol. 2023 Mar;38(1):e2023006-0. doi: 10.5620/eaht.2023006. Epub 2023 Mar 23.
Recent studies reported bisphenol A (BPA) and propyl paraben (PrP) are found in human urine, blood, and breast milk samples as well as in food, packaging, socks, and clothes. This means that the two chemicals co-exist in consumer products, and humans are exposed simultaneously to the mixture chemicals. However, the studies on the mixture effects of the two chemicals on human health are not enough. This study was designed to elucidate the effects of orally administered PrP, BPA, and their mixture effects on the uterotrophic response using ovariectomized rats. In addition, the correlation between the uterotrophic response and tissue concentrations of the two chemicals was studied to investigate whether one chemical has any effect on the absorption, distribution, or excretion of the other chemical. Histopathology, hematology, and plasma biochemistry analysis were also performed to evaluate the chemicals' toxicological effects in the treated rats. Although a significant increase in uterus weight (absolute and relative) was observed in the positive chemical (17β-estradiol) treated group, there were no statistical differences in the uterus weight between the vehicle control and the chemical-treated groups. However, a slight increase in the endometrial glands and a change in the cuboidal to columnar epithelium of the endometrial epithelium were observed in the mixture-treated group. There was no significant toxicity in all treated groups by the hematology and plasma biochemistry analysis results. The results of tissue distribution showed that BPA was mostly detected in the liver while PrP was not detected in most tissues, and the BPA level was higher when the rats were treated with PrP than without PrP, suggesting that PrP may increase the absorption of BPA after oral administration.
最近的研究报告称，在人类尿液、血液和母乳样本以及食品、包装、袜子和衣服中发现了双酚A（BPA）和对羟基苯甲酸丙酯（PrP）。这意味着这两种化学物质共存于消费品中，人类同时暴露于混合化学物质中。然而，关于这两种化学物质对人类健康的混合影响的研究还不够。本研究旨在阐明口服PrP、BPA及其混合物对去卵巢大鼠子宫营养反应的影响。此外，研究了子宫营养反应与两种化学物质组织浓度之间的相关性，以调查一种化学物质是否对另一种化学物质的吸收、分布或排泄有任何影响。还进行了组织病理学、血液学和血浆生物化学分析，以评估化学物质对受试大鼠的毒理学影响。虽然在阳性化学物质（17β-雌二醇）处理组中观察到子宫重量（绝对和相对）显著增加，但载体对照组和化学物质处理组之间的子宫重量没有统计学差异。然而，在混合物处理组中观察到子宫内膜腺体略有增加，子宫内膜上皮的立方上皮变为柱状上皮。血液学和血浆生物化学分析结果显示，所有处理组均无明显毒性。组织分布结果表明，BPA主要在肝脏中检测到，而PrP在大多数组织中未检测到，并且大鼠经PrP处理时的BPA水平高于未经PrP处理时，这表明PrP可能会增加口服给药后BPA的吸收。
