Páll Emöke, Niculae Mihaela, Brudașcă Gheorghe F, Ravilov Rustam Kh, Șandru Carmen Dana, Cerbu Constantin, Olah Diana, Zăblău Sergiu, Potârniche Adrian Valentin, Spinu Marina, Duca Gheorghiță, Rusu Mariana, Rzewuska Magdalena, Vasiu Aurel
Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of Russia Academy of Sciences", Tatar Scientific Research Institute of Agriculture, 420088 Kazan, Russia.
Antibiotics (Basel). 2021 Mar 22;10(3):333. doi: 10.3390/antibiotics10030333.
Antimicrobial and multidrug-resistant bacteria are a major problem worldwide and, consequently, the surveillance of antibiotic-resistant bacteria and assessment of the dissemination routes are essential. We hypothesized that migratory birds, coming from various environments, would carry more numerous strains than sedentary species, with increased risk to be passed to their contacts or environment in habitats they transit or nest in. Similarly, we presumed that strains from migratory birds will show multidrug resistance. A total of 170 oral and rectal swabs were collected from wild birds captured in different locations of the Danube Delta (Malic, Sfantu-Gheorghe, Letea Forest) and processed using standardized selective media. strains were confirmed by serology and molecular methods and, subsequently, their susceptibility was evaluated. The prevalence of species by host species, habitat type, and location was interpreted. The isolated species were identified as 14.33%, 13.33% 12% 17.33%, 10.88% with and (16%) also being prevalent. Of the 76 spp. isolates, 18.42% were resistant towards at least three antimicrobials, and 81.57% demonstrated a multidrug resistance phenotype, including mainly penicillins, aminoglycosides, and macrolides. The results of the present study indicate higher numbers of strains in migratory (74.66%) than in sedentary birds (25.33%), confirming our hypothesis. Furthermore, the increased pathogenicity of spp. strains, isolated from wild migratory and sedentary birds, was confirmed by their increased multiple antibiotic resistance (MAR) index (0.09-0.81).
抗菌和耐多药细菌是全球范围内的一个主要问题，因此，对抗生素耐药细菌的监测以及对传播途径的评估至关重要。我们假设，来自各种环境的候鸟携带的菌株比留鸟更多，将其传播给它们在迁徙途中或筑巢栖息地接触的对象或环境的风险也更高。同样，我们推测候鸟携带的菌株会表现出耐多药特性。从多瑙河三角洲不同地点（马利克、圣乔治、莱特亚森林）捕获的野生鸟类中总共采集了170份口腔和直肠拭子，并使用标准化的选择性培养基进行处理。通过血清学和分子方法确认菌株，随后评估其敏感性。分析了宿主物种、栖息地类型和地点对物种流行率的影响。分离出的物种分别为14.33%、13.33%、12%、17.33%、10.88%，和（16%）也很普遍。在76株分离株中，18.42%对至少三种抗菌药物耐药，81.57%表现出耐多药表型，主要包括青霉素、氨基糖苷类和大环内酯类。本研究结果表明，候鸟（74.66%）中的菌株数量高于留鸟（25.33%），证实了我们的假设。此外，从野生候鸟和留鸟中分离出的菌株多重抗生素耐药（MAR）指数增加（0.09 - 0.81），证实了其致病性增强。
