西格玛因子的结构分析

Collins Katherine M, Evans Nicola J, Torpey James H, Harris Jonathon M, Haynes Bethany A, Camp Amy H, Isaacson Rivka L

Department of Chemistry, King's College London, Britannia House, 7 Trinity Street, London SE1 1DB, UK.

Department of Biological Sciences, Mount Holyoke College, 50 College Street, South Hadley, MA 01075, USA.

Microorganisms. 2023 Apr 20;11(4):1077. doi: 10.3390/microorganisms11041077.

Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, , and present an X-ray crystal structure of a region of SigE, a sigma factor that plays a critical role in the developmental process of spore formation.

细菌在其生命周期的不同阶段使用一系列σ因子来调节基因表达。由于σ因子存在许多内在无序区域，通过实验获得其全长原子水平结构具有挑战性。如今，AlphaFold已为大多数σ因子提供了合理的全长模型。在此，我们讨论了对模式生物中σ因子结构和功能的当前理解，并展示了SigE一个区域的X射线晶体结构，SigE是一种在孢子形成发育过程中起关键作用的σ因子。