Azevedo Lucas Santos, Fonseca Juliana Costa, Castro Ana Hortência Fonsêca, Farias Katyuce de Souza, Zanuncio Vanessa Samúdio Santos, Silva Denise Brentan, Lima Luciana Alves Rodrigues Dos Santos
Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei (UFSJ), Divinópolis, MG, Brazil.
Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
Nat Prod Res. 2025 May;39(9):2649-2657. doi: 10.1080/14786419.2024.2324375. Epub 2024 Feb 29.
Steud. is distributed in Central and South America, commonly named as 'salsaparilha' or 'japicanga'. In the present work, the chemical composition was determined, antioxidant and cytotoxic effects were evaluated for the ethanol extract (EE) and fractions from leaves. Fatty acid esters and phytol were characterised in the hexane (HEX) fraction. -glycosylated flavonols and flavones, caffeic acid derivatives, and steroidal saponins were annotated for EE, and dichloromethane (DCM), ethyl acetate (AC), and hydroethanol (HE) fractions. The samples contain flavonoids and phenolic compounds, and the AC fraction displayed the biggest concentration of these substances. Antioxidant potential was observed in all samples, being especially pronounced in the AC fraction by DPPH and FRAP assays, with IC values of 8.18 and 2.35 µg/mL, respectively. AC and HEX fractions showed 35% and 5% lethality at 1000 μg/mL, in the assay, and the other samples did not show a toxic effect.
Steud.分布于中美洲和南美洲，通常被称为“洋菝葜”或“japicanga”。在本研究中，对叶乙醇提取物（EE）及其馏分的化学成分进行了测定，并评估了其抗氧化和细胞毒性作用。在己烷（HEX）馏分中鉴定出脂肪酸酯和叶绿醇。对EE以及二氯甲烷（DCM）、乙酸乙酯（AC）和氢乙醇（HE）馏分中的β-糖基化黄酮醇和黄酮、咖啡酸衍生物和甾体皂苷进行了注释。样品中含有黄酮类化合物和酚类化合物，AC馏分中这些物质的浓度最高。所有样品均具有抗氧化潜力，通过DPPH和FRAP测定法，AC馏分中的抗氧化潜力尤为明显，IC值分别为8.18和2.35μg/mL。在MTT试验中，AC和HEX馏分在1000μg/mL时的致死率分别为35%和5%，其他样品未显示出毒性作用。
