Tang Shan-Shan, Lu Jin-Chun, Xu Yuan-Hua, Wang Jing, Hong Ren-Yun, Ge Yan-Mei, Liang Yuan-Jiao
Center for Reproductive Medicine, Zhongda Hospital Southeast University Nanjing Jiangsu China.
Health Sci Rep. 2023 Apr 27;6(5):e1217. doi: 10.1002/hsr2.1217. eCollection 2023 May.
Due to the rapid motility of the selected sperm, sperm parameters cannot be accurately determined by the manual method. So, the application of a computer-assisted sperm analysis system with a high frame rate (HFR-CASA, 85 Hz) in sperm selection is investigated.
A total of 177 semen samples were collected for sperm selection with density gradient centrifugation. Then, the manual method and HFR-CASA method will be used to observe and analyze the sperm concentration, motility, and percentage of progressively motile sperm (PR) of the selected sperm samples. The quality control of sperm concentration was performed with microballoons. Two selected sperm samples were analyzed 10 times repeatedly to evaluate the accuracy of the HFR-CASA.
The results of microballoons analyzed with the HFR-CASA were in control. The coefficients of variation of sperm concentration, motility, and PR from two selected sperm samples were all below 10%. The results of 177 selected sperm samples analyzed by the manual method and HFR-CASA showed that although there were significant positive correlations in sperm concentration, motility, and PR between them ( < 0.001), the manual method significantly underestimated sperm concentration ( = 0.002) but overestimated sperm motility and PR ( < 0.001). When sperm concentration was below 50 × 10/mL, the manual method significantly overestimated sperm concentration ( < 0.05). However, when sperm concentration was more than 100 × 10/mL, the manual method significantly underestimated sperm concentration ( < 0.001). Regardless of sperm concentration, the manual method consistently overestimated sperm motility and PR ( < 0.001). When sperm concentration was more than 20 × 10/mL, there was no correlation in sperm PR between them ( > 0.05). When sperm concentration was below 50 × 10/mL, the correct rate of captured sperm by the HFR-CASA was more than 98%.
The HFR-CASA method is more accurate than the manual method in analyzing the selected sperm samples.
由于所选精子运动速度快，手动方法无法准确测定精子参数。因此，研究了高帧率计算机辅助精子分析系统（HFR-CASA，85Hz）在精子选择中的应用。
共收集177份精液样本，采用密度梯度离心法进行精子选择。然后，使用手动方法和HFR-CASA方法观察和分析所选精子样本的精子浓度、活力和进行性运动精子（PR）百分比。用微球进行精子浓度的质量控制。对两份所选精子样本进行10次重复分析，以评估HFR-CASA的准确性。
用HFR-CASA分析的微球结果在控制范围内。两份所选精子样本的精子浓度、活力和PR的变异系数均低于10%。手动方法和HFR-CASA对177份所选精子样本的分析结果表明，尽管它们之间的精子浓度、活力和PR存在显著正相关（<0.001），但手动方法显著低估了精子浓度（=0.002），但高估了精子活力和PR（<0.001）。当精子浓度低于50×10/mL时，手动方法显著高估了精子浓度（<0.05）。然而，当精子浓度超过100×/mL时，手动方法显著低估了精子浓度（<0.001）。无论精子浓度如何，手动方法始终高估精子活力和PR（<0.001）。当精子浓度超过20×10/mL时，它们之间的精子PR无相关性（>0.05）。当精子浓度低于50×10/mL时，HFR-CASA捕获精子的正确率超过98%。
在分析所选精子样本时，HFR-CASA方法比手动方法更准确。
