高效单细胞电阻抗谱分析。

High-Efficiency Single-Cell Electrical Impedance Spectroscopy.

机构信息

State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China.

Anhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, Anhui, China.

出版信息

Methods Mol Biol. 2023;2644:81-97. doi: 10.1007/978-1-0716-3052-5_6.

DOI:10.1007/978-1-0716-3052-5_6

PMID:37142917

Abstract

Single-cell impedance measurement is label free and noninvasive in characterizing the electrical properties of single cells. At present, though widely used for impedance measurement, electrical impedance flow cytometry (IFC) and electrical impedance spectroscopy (EIS) are used alone for most microfluidic chips. Here, we describe high-efficiency single-cell electrical impedance spectroscopy, which combines in one chip the IFC and EIS techniques for high-efficiency single-cell electrical property measurement. We envision that the strategy of combining IFC and EIS provides a new thought in the efforts to enhance the efficiency of electrical property measurement for single cells.

摘要

单细胞阻抗测量具有标记自由和非侵入性的特点，可用于描述单细胞的电学特性。目前，尽管广泛用于阻抗测量，但在大多数微流控芯片中，电阻抗流式细胞术（IFC）和电阻抗谱（EIS）技术通常是单独使用的。在这里，我们描述了高效的单细胞电阻抗谱，它将 IFC 和 EIS 技术结合在一个芯片中，用于高效的单细胞电特性测量。我们设想，将 IFC 和 EIS 相结合的策略为提高单细胞电特性测量效率提供了一种新的思路。