Bergh S T, Koziel M G, Huang S C, Thomas R A, Gilley D P, Siegel A
Nucleic Acids Res. 1985 Dec 9;13(23):8507-18. doi: 10.1093/nar/13.23.8507.
The nucleotide sequence of the smaller genomic strand (RNA-2) of the bipartite tobacco rattle virus (CAM strain) has been determined. RNA-2 is capped at the 5' terminus and contains 1799 nucleotide residues. There is a single 223 codon long open reading frame extending from nucleotide 574 to 1242 which designates a protein of Mr 23,654. The derived amino acid composition, in percent, matches that previously determined for the virus capsid protein. The long open reading frame is flanked by 5' and 3' untranslated regions of 573 and 554 nucleotides, respectively. The 5' leader sequence contains two different sets of direct repeats, one of 119 nucleotides and the other of 76. It also contains 13 apparently unused AUG codons, four of which lie in the same frame as the capsid protein cistron. The 3' terminal sequence of RNA-2 is identical to that of the larger genomic strand (RNA-1) for 459 nucleotides.
已确定双分体烟草脆裂病毒（CAM株系）较小基因组链（RNA - 2）的核苷酸序列。RNA - 2在5'末端有帽结构，包含1799个核苷酸残基。有一个从核苷酸574延伸至1242的单一223密码子长的开放阅读框，它编码一个Mr 23,654的蛋白质。推导的氨基酸组成百分比与先前确定的病毒衣壳蛋白相匹配。这个长开放阅读框两侧分别是573和554个核苷酸的5'和3'非翻译区。5'前导序列包含两组不同的直接重复序列，一组119个核苷酸，另一组76个核苷酸。它还包含13个明显未使用的AUG密码子，其中四个与衣壳蛋白顺反子处于同一阅读框。RNA - 2的3'末端序列与较大基因组链（RNA - 1）的459个核苷酸相同。
