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烟草脆裂病毒PSG株系的基因组结构分析

Analysis of the genome structure of tobacco rattle virus strain PSG.

作者信息

Cornelissen B J, Linthorst H J, Brederode F T, Bol J F

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):2157-69. doi: 10.1093/nar/14.5.2157.

DOI:10.1093/nar/14.5.2157
PMID:3960718
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339649/
Abstract

The sequence of the 3'-terminal 2077 nucleotides of genomic RNA 1 and the complete sequence of genomic RNA 2 of tobacco rattle virus (TRV, strain PSG) has been deduced. RNA 2 (1905 nucleotides) contains a single open reading frame for the viral coat protein (209 amino acids), flanked by 5'- and 3'-noncoding regions of 570 and 708 nucleotides, respectively. A subgenomic RNA (RNA 4) was found to lack the 5'-terminal 474 nucleotides of RNA 2 and is the putative messenger for coat protein. The deduced RNA 1 sequence contains the 3'-terminal part of a reading frame that probably corresponds to the TRV 170K protein and reading frames for a 29K protein and a 16K protein. Proteins encoded by the first two reading frames show significant amino acid sequence homology with corresponding proteins encoded by tobacco mosaic virus. Subgenomic RNAs 3 (1.6 kb) and 5 (0.7 kb) were identified as the putative messengers for the 29K and 16K proteins, respectively. At their 3'-termini all PSG-RNAs have an identical sequence of 497 nucleotides; at the 5'-termini homology is limited to 5 to 10 bases.

摘要

已推导得出烟草脆裂病毒（TRV，PSG株系）基因组RNA 1的3'末端2077个核苷酸序列以及基因组RNA 2的完整序列。RNA 2（1905个核苷酸）包含一个编码病毒外壳蛋白（209个氨基酸）的单一开放阅读框，其两侧分别是570个核苷酸的5'非编码区和708个核苷酸的3'非编码区。发现一种亚基因组RNA（RNA 4）缺少RNA 2的5'末端474个核苷酸，是外壳蛋白的推定信使RNA。推导得出的RNA 1序列包含一个阅读框的3'末端部分，该阅读框可能对应于TRV 170K蛋白，以及一个29K蛋白和一个16K蛋白的阅读框。前两个阅读框编码的蛋白质与烟草花叶病毒编码的相应蛋白质显示出显著的氨基酸序列同源性。亚基因组RNA 3（1.6 kb）和RNA 5（0.7 kb）分别被鉴定为29K和16K蛋白的推定信使RNA。在其3'末端，所有PSG-RNA都有一个497个核苷酸的相同序列；在5'末端，同源性仅限于5到10个碱基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef47/339649/13ae12c7c76b/nar00274-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef47/339649/13ae12c7c76b/nar00274-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef47/339649/13ae12c7c76b/nar00274-0231-a.jpg

相似文献

1
Analysis of the genome structure of tobacco rattle virus strain PSG.烟草脆裂病毒PSG株系的基因组结构分析
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2
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引用本文的文献

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Sci Rep. 2016 Oct 3;6:34482. doi: 10.1038/srep34482.
2
Function of the 30 kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication.烟草花叶病毒 30kD 蛋白的功能：参与细胞间运动和复制的非必需性。
EMBO J. 1987 Sep;6(9):2557-63. doi: 10.1002/j.1460-2075.1987.tb02544.x.
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本文引用的文献

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Tobacco mosaic virus: model for structure and function of a simple virus.烟草花叶病毒：简单病毒结构与功能的模型
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Nucleotide sequence of tobacco mosaic virus RNA.烟草花叶病毒RNA的核苷酸序列。
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Nucleic Acids Res. 1986 Jun 11;14(11):4673-82. doi: 10.1093/nar/14.11.4673.
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tRNA-like properties of tobacco rattle virus RNA.烟草脆裂病毒RNA的类tRNA特性
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Probable reassortment of genomic elements among elongated RNA-containing plant viruses.含RNA的伸长型植物病毒基因组元件间可能的重配。
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Pea early browning virus RNA1 encodes four polypeptides including a putative zinc-finger protein.豌豆早褐病毒RNA1编码四种多肽，包括一种假定的锌指蛋白。
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New M13 vectors for cloning.用于克隆的新型M13载体。
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Complete nucleotide sequence of alfalfa mosaic virus RNA 1.苜蓿花叶病毒RNA 1的完整核苷酸序列。
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