Department of Bioengineering and Biotechnology BIT Mesra, Ranchi, Jharkhand, 835215, India.
Department of Plant Sciences and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Curr Microbiol. 2023 May 5;80(6):202. doi: 10.1007/s00284-023-03313-0.
Acinetobacter species is currently ranked as high-priority pathogen for their extraordinary ability to become resistant to almost all existing antibiotics. The diverse range of effectors secreted by Acinetobacter spp. constitutes a significant proportion of the virulence arsenal. Therefore, our study aims to characterize the secretome of Acinetobacter pittii S-30. Analysis of extracellular secreted proteins of A. pittii S-30 revealed the presence of transporter proteins, outer membrane proteins, molecular chaperones, porins, and several proteins of unknown function. Additionally, proteins related to metabolism, as well as those involved in gene expression and protein translation, type VI secretion system (T6SS) proteins, and stress response-related proteins were also identified in the secretome. The comprehensive analysis of secretome revealed putative protein antigens which could elicit substantial immune response. The limited availability of effective antibiotics and the worldwide growth of secretome data make this approach appealing in the development of effective vaccines against Acinetobacter and other bacterial pathogens.
不动杆菌属目前被列为高优先级病原体，因为它们具有几乎对抗所有现有抗生素的耐药能力。不动杆菌属分泌的多种效应子构成了毒力武器库的重要组成部分。因此，我们的研究旨在表征鲍曼不动杆菌 S-30 的分泌组。对 A. pittii S-30 细胞外分泌蛋白的分析表明，存在转运蛋白、外膜蛋白、分子伴侣、孔蛋白和几种未知功能的蛋白。此外，在分泌组中还鉴定到与代谢相关的蛋白，以及参与基因表达和蛋白质翻译、VI 型分泌系统（T6SS）蛋白和应激反应相关蛋白的蛋白。对分泌组的全面分析揭示了可能引发强烈免疫反应的潜在蛋白抗原。有效的抗生素的有限供应和全球范围内分泌组数据的增长，使得这种方法在开发针对不动杆菌和其他细菌病原体的有效疫苗方面具有吸引力。
