评估潜在抗衰老药物处理后秀丽隐杆线虫健康寿命的方案。
Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment.
作者信息
Xiao Yi, Zhang Li, Liu Yun
机构信息
Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China; College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou 563000, China.
Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China; College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou 563000, China.
出版信息
STAR Protoc. 2023 May 5;4(2):102285. doi: 10.1016/j.xpro.2023.102285.
The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization, drug treatment, and lifespan determination from the survivorship curve. We also detail assessment of locomotory ability through body bend rate and measurement of lipofuscin fluorescence to quantify age pigment in the worm intestine. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022)..
线虫秀丽隐杆线虫已被开发成为研究衰老及衰老相关疾病的重要遗传模型。在此，我们介绍一种在潜在抗衰老药物处理后评估秀丽隐杆线虫健康寿命的方案。我们描述了秀丽隐杆线虫同步化、药物处理以及根据生存曲线确定寿命的步骤。我们还详细说明了通过身体弯曲率评估运动能力以及测量脂褐素荧光以量化线虫肠道中衰老色素的方法。有关本方案使用和实施的完整详细信息，请参阅Xiao等人（2022年）的文章。
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