Speirs R S, Benson R W, Roberts D W
Infect Immun. 1979 Mar;23(3):675-80. doi: 10.1128/iai.23.3.675-680.1979.
Pertussis vaccine (PV) or diphtheria toxoid-PV-tetanus toxoid (DPT) altered the antibody response of BALB/c female mice to type III pneumococcal polysaccharide antigen (S3). The key factor affecting the magnitude of the response to S3 was the route of injection of PV or DPT, whereas the route of injection of S3 was not crucial. Subcutaneous injections of DPT augmented the antibody response to low, optimal, and tolerogenic (high) doses of S3 injected either subcutaneously or intraperitoneally. This enhancement was persistent and was observed both when S3 and DPT were mixed and injected subcutaneously and when S3 and DPT were injected concurrently at separate subcutaneous sites. When either PV or DPT was injected intraperitoneally, the antibody response to subcutaneously or intraperitoneally injected S3 was significantly decreased. These experiments demonstrate a dichotomy of effect dependent on the route of administration of PV. Most studies in mice utilizing PV employ the intraperitoneal injection route, and it is important to consider whether PV treatment by this route may have unique effects. Our data suggest that intraperitoneal injection of PV suppresses B cells, possibly by influencing regulatory cell function.
百日咳疫苗（PV）或白喉类毒素-百日咳疫苗-破伤风类毒素（DPT）改变了BALB/c雌性小鼠对Ⅲ型肺炎球菌多糖抗原（S3）的抗体反应。影响对S3反应强度的关键因素是PV或DPT的注射途径，而S3的注射途径并不关键。皮下注射DPT增强了对皮下或腹腔注射的低剂量、最佳剂量和致耐受性（高剂量）S3的抗体反应。这种增强是持续的，在S3和DPT混合后皮下注射以及S3和DPT在不同皮下部位同时注射时均观察到。当PV或DPT腹腔注射时，对皮下或腹腔注射S3的抗体反应显著降低。这些实验证明了依赖于PV给药途径的效应二分法。在小鼠中利用PV的大多数研究采用腹腔注射途径，重要的是要考虑通过该途径进行PV治疗是否可能有独特的效应。我们的数据表明，腹腔注射PV可能通过影响调节细胞功能来抑制B细胞。
