College of Enology and Horticulture, Ningxia University, Yinchuan 750021, China.
Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan 750021, China.
Int J Mol Sci. 2023 May 4;24(9):8236. doi: 10.3390/ijms24098236.
Powdery mildew is a serious problem in tomato production; therefore, the PM-resistant tomato inbred line, '63187', and the susceptible tomato variety, 'Moneymaker (MM)', were used as experimental materials for the combined analysis of transcriptome and widely targeted metabolome on tomato leaves at 0 h post inoculation (hpi), 12 hpi, and 48 hpi. The results indicated that 276 genes were expressed in all treatments, and the K-means cluster analysis showed that these genes were divided into eight classes in '63187' and ten classes in 'MM'. KEGG enrichment showed that amino acid metabolism, signal transduction, energy metabolism, and other secondary metabolites biosynthesis pathways were significantly enriched. Interestingly, the analysis of WRKY family transcription factors (TFs) showed that the expression of four TFs in '63187' increased with no obvious change in 'MM'; and the expression of one TF in 'MM' increased with no obvious change in '63187'. The combined analysis revealed that both phenylpropanoid biosynthesis and flavonoid biosynthesis pathways were enriched in '63187' and 'MM'. In '63187', six metabolites involved in this pathway were downregulated, and four genes were highly expressed, while in 'MM', three metabolites were upregulated, four metabolites were downregulated, and ten genes were highly expressed. These metabolites and genes might be candidates for PM resistance or susceptibility in subsequent studies. These results provide favorable molecular information for the study of the different resistances of tomatoes to PM, and they provide a basis for the breeding of tomato varieties resistant to PM.
白粉病是番茄生产中的一个严重问题；因此，选用抗白粉病的番茄自交系‘63187’和易感番茄品种‘Moneymaker（MM）’作为实验材料，对番茄叶片进行了转录组和广泛靶向代谢组的联合分析，接种后 0 小时（hpi）、12 小时（hpi）和 48 小时（hpi）。结果表明，在所有处理中表达了 276 个基因，K-means 聚类分析表明，这些基因在‘63187’中分为 8 类，在‘MM’中分为 10 类。KEGG 富集表明，氨基酸代谢、信号转导、能量代谢和其他次生代谢物生物合成途径显著富集。有趣的是，WRKY 家族转录因子（TFs）分析表明，在‘63187’中，四个 TF 的表达随‘MM’无明显变化而增加；在‘MM’中，一个 TF 的表达随‘63187’无明显变化而增加。联合分析表明，苯丙烷生物合成和黄酮类生物合成途径在‘63187’和‘MM’中均有富集。在‘63187’中，该途径的 6 种代谢物下调，4 种基因高度表达，而在‘MM’中，3 种代谢物上调，4 种代谢物下调，10 种基因高度表达。这些代谢物和基因可能是番茄对白粉病不同抗性的候选基因，为后续研究提供了有利的分子信息。
