Harbin Normal University, Harbin, 150025, China.
Funct Integr Genomics. 2024 Jan 18;24(1):14. doi: 10.1007/s10142-024-01287-1.
Cytochrome P450s are a large family of protein-encoding genes in plant genomes, many of which have not yet been comprehensively characterized. Here, a novel P450 gene, CYP82D47, was isolated and functionally characterized from cucumber (Cucumis sativus L.). Quantitative real-time reverse-transcription polymerase chain reaction analysis revealed that CYP82D47 expression was triggered by salicylic acid (SA) and ethephon (ETH). Expression analysis revealed a correlation between CYP82D47 transcript levels and plant defense responses against powdery mildew (PM) and Fusarium oxysporum f. sp. cucumerinum (Foc). Although no significant differences were observed in disease resistance between CYP82D47-RNAi and wild-type cucumber, overexpression (OE) of CYP82D47 enhanced PM and Foc resistance in cucumber. Furthermore, the expression levels of SA-related genes (PR1, PR2, PR4, and PR5) increased in CYP82D47-overexpressing plants 7 days post fungal inoculation. The levels of ETH-related genes (EIN3 and EBF2) were similarly upregulated. The observed enhanced resistance was associated with the upregulation of SA/ETH-signaling-dependent defense genes. These findings indicate the crucial role of CYP82D47 in pathogen defense in cucumber. CYP82D47-overexpressing cucumber plants exhibited heightened susceptibility to both diseases. The study results offer important insights that could aid in the development of disease-resistant cucumber cultivars and elucidate the molecular mechanisms associated with the functions of CYP82D47.
细胞色素 P450s 是植物基因组中一个庞大的蛋白质编码基因家族，其中许多尚未得到全面表征。在这里，从黄瓜（Cucumis sativus L.）中分离并功能表征了一种新型 P450 基因 CYP82D47。定量实时逆转录聚合酶链反应分析显示，CYP82D47 的表达受水杨酸（SA）和乙烯利（ETH）触发。表达分析显示 CYP82D47 转录本水平与植物对白粉病（PM）和尖孢镰刀菌黄瓜专化型（Foc）的防御反应之间存在相关性。尽管 CYP82D47-RNAi 和野生型黄瓜之间在抗病性方面没有观察到显著差异，但 CYP82D47 的过表达（OE）增强了黄瓜对 PM 和 Foc 的抗性。此外，SA 相关基因（PR1、PR2、PR4 和 PR5）在 CYP82D47 过表达植物中真菌接种后 7 天表达水平增加。ETH 相关基因（EIN3 和 EBF2）也被类似地上调。观察到的增强抗性与 SA/ETH 信号依赖性防御基因的上调有关。这些发现表明 CYP82D47 在黄瓜的病原体防御中起着至关重要的作用。过表达 CYP82D47 的黄瓜植物对这两种疾病表现出更高的敏感性。该研究结果提供了重要的见解，可有助于开发抗病性黄瓜品种，并阐明与 CYP82D47 功能相关的分子机制。
