Mei Nan, Postec Anne, Erauso Gael, Joseph Manon, Pelletier Bernard, Payri Claude, Ollivier Bernard, Quéméneur Marianne
Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
Centre IRD de Nouméa, 101 Promenade Roger Laroque, BP A5 - 98848 Nouméa cedex, New Caledonia.
Int J Syst Evol Microbiol. 2016 Nov;66(11):4464-4470. doi: 10.1099/ijsem.0.001375. Epub 2016 Aug 4.
A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3bT, grew at temperatures from 30 to 43 °C (optimum 37 °C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0-0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3bT utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was used as an electron acceptor and converted to succinate. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate, nitrite, FeCl3, Fe(III)-citrate, Fe(III)-EDTA, chromate, arsenate, selenate and DMSO were not used as terminal electron acceptors. The G+C content of the genomic DNA was 33.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family Clostridiaceae, order Clostridiales within the phylum Firmicutes. Strain 3bT was most closely related to 'Alkaliphilus hydrothermalis' FatMR1T (92.2 % 16S rRNA gene sequence similarity), and was positioned approximately equidistantly between the genera Alkaliphilus, Anaerovirgula and Natronincola. On the basis of phylogenetic, genetic, chemotaxonomic and physiological properties, strain 3bT is proposed to represent a novel species of a new genus, for which the name Serpentinicella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Serpentinicella alkaliphila is 3bT (=DSM 100013T=JCM 30645T).
从新喀里多尼亚普洛尼湾的一个含碳热液烟囱中分离出一种新型厌氧、嗜碱、革兰氏染色阳性、产芽孢细菌。这种细菌被命名为菌株3bT，在30至43 °C（最适温度37 °C）的温度范围内以及pH值在7.8至10.1（最适pH值9.5）之间生长。生长不需要添加NaCl（最适浓度0-0.2 %，w/v），但在高达4 %的浓度下也能耐受。生长需要酵母提取物。菌株3bT利用巴豆酸盐、乳酸盐和丙酮酸盐，但不利用糖类。巴豆酸盐歧化为乙酸盐和丁酸盐。乳酸盐歧化为乙酸盐和丙酸盐。丙酮酸盐降解为乙酸盐加微量氢气。添加富马酸盐可促进菌株在乳酸盐上的生长，富马酸盐用作电子受体并转化为琥珀酸盐。硫酸盐、硫代硫酸盐、元素硫、亚硫酸盐、硝酸盐、亚硝酸盐、FeCl3、柠檬酸铁(III)、乙二胺四乙酸铁(III)、铬酸盐、砷酸盐、硒酸盐和二甲基亚砜均未用作末端电子受体。基因组DNA的G+C含量为33.2 mol%。基于16S rRNA基因序列的系统发育分析表明，该分离株是厚壁菌门梭菌目梭菌科的成员。菌株3bT与“嗜碱嗜热栖热菌”FatMR1T关系最为密切（16S rRNA基因序列相似性为92.2 %），并且在嗜碱栖热菌属、厌氧游动菌属和嗜盐栖热菌属之间的位置大致等距。基于系统发育、遗传、化学分类和生理特性，提议菌株3bT代表一个新属的一个新物种，为此提出新属名嗜碱蛇形菌属（Serpentinicella），新种名嗜碱嗜碱蛇形菌（Serpentinicella alkaliphila）。嗜碱嗜碱蛇形菌的模式菌株为3bT（=DSM 100013T=JCM 30645T）。
