Assié L K, Deleu M, Arnaud L, Paquot M, Thonart P, Gaspar Ch, Haubruge E
Unité de Zoolgie générale et appliquée, Faculté universitaire des Sciences agronomiques de Gembloux, B-5030 Gembloux, Belgium.
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2002;67(3):647-55.
Surfactin C14, surfactin C15, and iturin C15 are lipopeptides purified from Bacillus subtilis (S499 strain). They were incorporated to artificial diet of the fruit fly Drosophila melanogaster (Meigen) (Diptera, Drosophilidae) to assess their potential insecticide activity. Surfactins with long fatty acid chain (C14 and C15) showed insecticide effect on the fruit fly, D. melanogaster. On the contrary, iturin was not toxic to fruit fly D. melanogaster. At 100 ppm, surfactin C14 and C15 showed respectively 85.4 and 92.6% adults mortality after one-day exposure. F1 progeny fly emergence inhibition by C14 and C15 were respectively 79.8% and 91.3%. To check whether the biocide activity of lipopeptides was due to their surface-active properties, detergent Triton X100, SDS, CTAB and Tween 80 were tested. No adult mortality was recorded with the detergents but Triton X100 and SDS showed F1 progeny emergence inhibition similar to that of surfactins. We showed that there was a dose-response activity with surfactin C15.
表面活性素C14、表面活性素C15和伊枯草菌素C15是从枯草芽孢杆菌（S499菌株）中纯化得到的脂肽。将它们添加到果蝇（黑腹果蝇，Meigen）（双翅目，果蝇科）的人工饲料中，以评估其潜在的杀虫活性。具有长脂肪酸链（C14和C15）的表面活性素对果蝇黑腹果蝇显示出杀虫效果。相反，伊枯草菌素对果蝇黑腹果蝇无毒。在100 ppm时，表面活性素C14和C15在暴露一天后分别显示出85.4%和92.6%的成虫死亡率。C14和C15对F1代子代果蝇羽化的抑制率分别为79.8%和91.3%。为了检查脂肽的杀生物活性是否归因于其表面活性特性，对去污剂Triton X100、SDS、CTAB和吐温80进行了测试。使用这些去污剂未记录到成虫死亡，但Triton X100和SDS显示出与表面活性素类似的对F1代子代羽化的抑制作用。我们表明表面活性素C15存在剂量反应活性。
