Unit of Biology and Genetics of Movement, Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Italy.
Physical Exercise and Sport Sciences Unit, Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", Rome, Italy.
Free Radic Biol Med. 2023 Aug 1;204:266-275. doi: 10.1016/j.freeradbiomed.2023.05.007. Epub 2023 May 13.
Considering the role of redox homeostasis in exercise-induced signaling and adaptation, this study focuses on the exercise training-related intercellular communication of redox status mediated by circulating extracellular vesicles (EVs). 19 healthy young males were divided into trained (TG, 7) and untrained (UG, 12) subjects based on their VO. The UG subjects were further randomly distributed in experimental (UG, N = 7) and control (UG, N = 5) groups. The steady state of plasma EVs in TG and UG have been characterized for total number and size, as well as cargo redox status (antioxidants, transcription factors, HSPs) before, 3 and 24 h after a single bout of aerobic exercise (30', 70% HRM). Plasma EVs from UG and UG have been further characterized after 24 h from the last session of a 5-day consecutive aerobic training or no training, respectively. No differences were detected in the EVs' size and distribution at baseline in TG and UG (p>0.05), while the EVs cargo of UG showed a significantly higher concentration of protein carbonyl, Catalase, SOD2, and HSF1 compared to TG (p<0.05). 5 days of consecutive aerobic training in UG did not determine major changes in the steady-state number and size of EVs. The post-training levels of protein carbonyl, HSF1, Catalase, and SOD2 in EVs cargo of UG resulted significantly lower compared with UG before training and UG, resembling the steady-state levels in circulating EVs of TG subjects. Altogether, these preliminary data indicate that individual aerobic capacity influences the redox status of circulating EVs, and that short-term aerobic training impacts the steady-state redox status of EVs. Taking this pilot study as a paradigm for physio-pathological stimuli impacting redox homeostasis, our results offer new insights into the utilization of circulating EVs as biomarkers of exercise efficacy and of early impairment of oxidative-stress related diseases.
考虑到氧化还原稳态在运动诱导的信号转导和适应中的作用，本研究侧重于运动训练相关的细胞间通讯，即由循环细胞外囊泡 (EVs) 介导的氧化还原状态。根据 VO，将 19 名健康年轻男性分为训练组 (TG，7 人) 和未训练组 (UG，12 人)。UG 组进一步随机分为实验组 (UG，N=7) 和对照组 (UG，N=5)。在单次有氧运动 (30'，70% HRM) 前、后 3 和 24 小时，对 TG 和 UG 中的 EV 总数和大小以及 EV 货物的氧化还原状态 (抗氧化剂、转录因子、HSPs) 进行了特征描述。进一步在连续 5 天的有氧运动最后一次训练后 24 小时对 UG 和 UG 的 EV 进行了特征描述。在 TG 和 UG 中，EVs 的大小和分布在基线时没有差异 (p>0.05)，而 UG 的 EVs 货物中蛋白羰基、过氧化氢酶、SOD2 和 HSF1 的浓度明显高于 TG (p<0.05)。在 UG 中进行连续 5 天的有氧运动训练并没有导致 EVs 数量和大小的稳态发生重大变化。与训练前和 UG 相比，UG 中 EVs 货物的蛋白羰基、HSF1、过氧化氢酶和 SOD2 的训练后水平显著降低，与 TG 受试者循环 EVs 的稳态水平相似。总之，这些初步数据表明，个体有氧能力影响循环 EVs 的氧化还原状态，短期有氧运动训练影响 EVs 的稳态氧化还原状态。将这项初步研究作为影响氧化还原平衡的生理病理刺激的范例，我们的结果为将循环 EVs 作为运动效果的生物标志物和氧化应激相关疾病早期损伤的生物标志物提供了新的见解。
