Department of Pathology, University of Iowa, Iowa City, USA.
Program in Biomedical Sciences, Rackham Graduate School, University of Michigan, Ann Arbor, MI, USA.
Environ Toxicol Pharmacol. 2023 Jun;100:104149. doi: 10.1016/j.etap.2023.104149. Epub 2023 May 15.
The widespread use of glyphosate, a broad-spectrum herbicide, has resulted in significant human exposure, and recent studies have challenged the notion that glyphosate is safe for humans. Although the link between disease states and glyphosate exposure is increasingly appreciated, the mechanistic links between glyphosate and its toxic effects on human health are poorly understood. Recent studies have suggested that glyphosate may cause toxicity through modulation of the gut microbiome, but evidence for glyphosate-induced gut dysbiosis and its effect on host physiology at doses approximating the U.S. Acceptable Daily Intake (ADI = 1.75 mg/kg body weight) is limited. Here, utilizing shotgun metagenomic sequencing of fecal samples from C57BL/6 J mice, we show that glyphosate exposure at doses approximating the U.S. ADI significantly impacts gut microbiota composition. These gut microbial alterations were associated with effects on gut homeostasis characterized by increased proinflammatory CD4IL17A T cells and Lipocalin-2, a known marker of intestinal inflammation.
草甘膦是一种广谱除草剂，被广泛使用，导致人类大量接触，最近的研究对草甘膦对人类安全的观点提出了挑战。尽管疾病状态与草甘膦暴露之间的联系越来越受到重视，但草甘膦与人类健康毒性之间的机制联系仍知之甚少。最近的研究表明，草甘膦可能通过调节肠道微生物组引起毒性，但在接近美国可接受日摄入量 (ADI = 1.75mg/kg 体重) 的剂量下，草甘膦诱导的肠道菌群失调及其对宿主生理学的影响的证据有限。在这里，我们利用 C57BL/6J 小鼠粪便样本的 shotgun 宏基因组测序表明，接近美国 ADI 的草甘膦暴露剂量显著影响肠道微生物群落组成。这些肠道微生物的改变与肠道内稳态的影响有关，其特征是促炎 CD4IL17A T 细胞和脂联素-2（一种已知的肠道炎症标志物）增加。
