Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Chinese Medicine, Yuhua Road, Chenggong District, Kunming, 650500, Yunnan, People's Republic of China.
Curr Microbiol. 2023 May 18;80(7):215. doi: 10.1007/s00284-023-03290-4.
The wild resources of Psammosilene tunicoides have decreased sharply because of the long-term mining and excavation, which has led to the increased demand for its artificial cultivation. However, root rot represents a significant obstacle leading to a poor quality and product of P. tunicoides. Previous reports have not focused on root rot in P. tunicoides. Therefore, this study explores the rhizospheric and root endophytic microbial community structure and composition of healthy and root rot P. tunicoides to understand the mechanism underlying root rot. The properties of the rhizosphere soil were assessed using physiochemical methods, and the bacterial and fungal populations were studied through amplicon sequencing of the 16S rRNA genes and ITS regions in the root and soil. Compared to healthy samples, the pH, hydrolysis N, available P, and available K were significantly decreased in the diseased samples while the organic matter and total organic carbon were significantly increased in the diseased samples. Redundancy analysis (RDA) showed that soil environmental factors are related to changes in the root and rhizosphere soil microbial community of P. tunicoides indicating that the physiochemical properties of soil affect plant health. Alpha diversity analysis showed that the microbial communities of healthy and diseased samples were similar. Some bacterial and fungal genera were significantly increased or decreased (P < 0.05) in diseased P. tunicoides, and certain microbial factors that antagonized root rot were further explored. This study provides an abundant microbial resource for future studies and contributes to improving soil quality and P. tunicoides agricultural production.
由于长期的开采和挖掘，球果堇菜的野生资源急剧减少，导致对其人工栽培的需求增加。然而，根腐病是导致球果堇菜质量和产量下降的主要障碍。以前的报告没有关注球果堇菜的根腐病。因此，本研究探讨了健康和根腐病球果堇菜的根际和根内生微生物群落结构和组成，以了解根腐病的发生机制。采用理化方法评估根际土壤性质，通过对根和土壤中 16S rRNA 基因和 ITS 区的扩增子测序研究细菌和真菌种群。与健康样本相比，患病样本的 pH 值、水解 N、有效 P 和有效 K 显著降低，而有机质和总有机碳显著增加。冗余分析（RDA）表明，土壤环境因素与球果堇菜根和根际土壤微生物群落的变化有关，表明土壤理化性质影响植物的健康。α多样性分析表明，健康和患病样本的微生物群落相似。在患病的球果堇菜中，一些细菌和真菌属显著增加或减少（P < 0.05），并进一步探讨了某些拮抗根腐病的微生物因素。本研究为未来的研究提供了丰富的微生物资源，有助于提高土壤质量和球果堇菜的农业生产。
