Cole D E, Boucher M J
Clin Chem. 1986 Jul;32(7):1375-8.
We compared concentrations of chloride and sulfate in sweat obtained by use of the Macroduct capillary-coil collection device with results obtained by the conventional absorbent filter pad technique. Samples obtained with the device weighed less than those obtained conventionally, but sweat chloride concentrations were not significantly different. To assess analysis of trace anions, we used inorganic sulfate as a prototype. Background contamination, a problem with the filter pads, was negligible with the Macroduct collector. However, with the Macroduct device, sulfate concentrations were nominally higher than with the conventional pads (105 +/- 6 vs 88 +/- 5 mumol/L) and showed no dependence of sulfate concentration on sweat rate. Subtraction of the significant "background" SO4 concentration obtained with blanks (i.e., unused filter pads) is a likely source of error in the conventional method. We consider the Macroduct device useful for study of trace constituents of human sweat.
我们将使用Macroduct毛细管线圈收集装置获取的汗液中氯化物和硫酸盐的浓度，与采用传统吸收性滤垫技术所获结果进行了比较。该装置获取的样本重量比传统方法获取的样本轻，但汗液氯化物浓度并无显著差异。为评估痕量阴离子的分析情况，我们将无机硫酸盐用作原型。背景污染是滤垫存在的一个问题，而Macroduct收集器的背景污染可忽略不计。然而，使用Macroduct装置时，硫酸盐浓度名义上高于传统滤垫（分别为105±6和88±5μmol/L），且未显示出硫酸盐浓度与出汗率有关。减去空白样本（即未使用的滤垫）所获得的显著“背景”SO4浓度，可能是传统方法中的一个误差来源。我们认为Macroduct装置对于研究人体汗液中的痕量成分很有用。
