Asadian Maryam, Yaghoubi Hashem, Mahmoudi Fariba, Haghighat Gollo Khadijeh
Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Galen Med J. 2022 Dec 13;11:e2219. doi: 10.31661/gmj.v11i.2219. eCollection 2022.
The process of steroidogenesis is crucial to the normal function of the ovaries. In individuals with polycystic ovary syndrome (PCOS), the activity of related enzymes in this process is disrupted. In the present study, the effect of trans-anethole was investigated on gene expression of steroidogenesis enzymes in PCOS model rats.
In this experimental study, thirty female rats were divided into six groups (n=5 per group). Fifteen PCOS rats in three groups received intraperitoneal injections of distilled water, 50, and 80 mg/kg of trans-anethole, respectively. Also, 15 intact rats in three groups received intraperitoneal injections of distilled water, 50, and 80 mg/kg trans-anethole. The expression of steroidogenesis genes was determined using real-time reverse transcription polymerase chain reaction.
The mRNA level of Cyp19 significantly increased in intact rats receiving 80 mg/kg trans-anethole compared to the control group. The Cyp19 level in PCOS groups was significantly reduced compared to the control group. The mRNA level of Cyp19 in PCOS groups that resived 50 or 80 mg/kg trans-anethole increased compared to PCOS rats, but this increase was not statistically significant. The mRNA level of Cyp17 did not significantly change in intact and PCOS rats that received trans-anethole compared to the control group.
Trans-anethole may improve PCOS complications due to its involvement in regulating steroidogenesis.
类固醇生成过程对卵巢的正常功能至关重要。在多囊卵巢综合征（PCOS）患者中，该过程中相关酶的活性受到破坏。在本研究中，研究了反式茴香脑对PCOS模型大鼠类固醇生成酶基因表达的影响。
在本实验研究中，30只雌性大鼠分为6组（每组n = 5）。三组中的15只PCOS大鼠分别接受腹腔注射蒸馏水、50和80 mg/kg反式茴香脑。另外，三组中的15只正常大鼠接受腹腔注射蒸馏水、50和80 mg/kg反式茴香脑。使用实时逆转录聚合酶链反应测定类固醇生成基因的表达。
与对照组相比，接受80 mg/kg反式茴香脑的正常大鼠中Cyp19的mRNA水平显著升高。与对照组相比，PCOS组中Cyp19水平显著降低。接受50或80 mg/kg反式茴香脑的PCOS组中Cyp19的mRNA水平与PCOS大鼠相比有所升高，但这种升高无统计学意义。与对照组相比，接受反式茴香脑的正常和PCOS大鼠中Cyp17的mRNA水平无显著变化。
反式茴香脑可能因其参与调节类固醇生成而改善PCOS并发症。
