反式茴香脑对大鼠多囊卵巢综合征所致组织病理学和代谢变化的保护作用
The Protective Effects of Trans-Anethole against Polycystic Ovary Syndrome Induced Histopathological and Metabolic Changes in Rat.
作者信息
Moradi Negahdari Faezeh, Hadjzadeh Mousa-Al-Reza, Gholamnezhad Zahra, Sohrabi Farzaneh, Samadi Noshahr Zahra
机构信息
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Int J Fertil Steril. 2022 Aug 21;16(3):192-199. doi: 10.22074/ijfs.2021.532941.1148.
Aim of the study was to evaluate the protective effects of trans-anethole, against polycystic ovary syndrome (PCOS) induced histopathological and biochemical changes in female Wister rats.
Materials and Methods: In this experimental study, forty-eight animals were randomly assigned into 6 groups: control; PCOS; PCOS+trans-anethole (20, 40, 80 mg/kg); and PCOS+metformin (300 mg/kg). Testosterone (1 mg/kg/day) was injected intraperitoneally for 35 days to induce PCOS. After PCOS induction, animals were treated by transanethole and metformin (30 days oral gavage). Finally, serum oxidative stress and insulin levels as well as histological changes in ovaries, kidneys and liver were evaluated.
Results: In PCOS group, the serum level of malondialdehyde (MDA) was 1.391 ± 0.18 mmol/L and significantly
increased (P=0.000) compared to the control group with the MDA level of 0.35 ± 0.08. Meanwhile the activity of
superoxide dismutase (SOD) and catalase (CAT), and total thiol levels were significantly decreased (P=0.000 for all
groups), compared to the control group. In the trans-anethole (80 mg/kg) treated group, insulin (P=0.000) and MDA
(P=0.000) levels were significantly decreased while total thiol (P=0.001) and activity of SOD (P=0.000) and CAT
(P=0.007) were significantly increased compared to the PCOS group. In the metformin treated group the insulin level
(P=0.03) decreased compared to the PCOS group. Histological evaluation showed multiple cysts in the ovarian tissue,
an increase in inflammatory cells in the liver, and a loss of order in the structure of the tubules and glomeruli of the
kidney in the PCOS group. Tissue damage was reduced in the trans-anethole treated group.
Conclusion: Tarns-anethole at a dose of 80 mg/kg improved metabolic status, oxidative stress, liver and kidney damage
as well as the cystic mass of ovarian tissue. To understand the exact protective effects of trans-anethole in PCOS,
more experimental or clinical studies are suggested.
本研究旨在评估反式茴香脑对多囊卵巢综合征（PCOS）诱导的雌性Wistar大鼠组织病理学和生化变化的保护作用。
材料与方法
在本实验研究中，48只动物被随机分为6组：对照组；PCOS组；PCOS + 反式茴香脑（20、40、80 mg/kg）组；以及PCOS + 二甲双胍（300 mg/kg）组。腹腔注射睾酮（1 mg/kg/天）35天以诱导PCOS。诱导PCOS后，动物接受反式茴香脑和二甲双胍治疗（口服灌胃30天）。最后，评估血清氧化应激和胰岛素水平以及卵巢、肾脏和肝脏的组织学变化。
结果
在PCOS组中，丙二醛（MDA）血清水平为1.391±0.18 mmol/L，与MDA水平为0.35±0.08的对照组相比显著升高（P = 0.000）。同时，与对照组相比，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性以及总硫醇水平显著降低（所有组P = 0.000）。在反式茴香脑（80 mg/kg）治疗组中，与PCOS组相比，胰岛素（P = 0.000）和MDA（P = 0.000）水平显著降低，而总硫醇（P = 0.001）以及SOD（P = 0.000）和CAT（P = 0.007）的活性显著增加。在二甲双胍治疗组中，与PCOS组相比胰岛素水平（P = 0.03）降低。组织学评估显示，PCOS组卵巢组织中有多个囊肿，肝脏中炎性细胞增加，肾脏肾小管和肾小球结构紊乱。反式茴香脑治疗组的组织损伤减轻。
结论
80 mg/kg剂量的反式茴香脑改善了代谢状态、氧化应激、肝脏和肾脏损伤以及卵巢组织的囊肿性肿块。为了解反式茴香脑在PCOS中的确切保护作用，建议进行更多的实验或临床研究。
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