DISCO Beamline, Synchrotron SOLEIL, BP 48, Gif sur Yvette 91192, France; University of Belgrade, Faculty of Physics, Studentski trg 12, Belgrade 11001, Serbia.
University of Belgrade, "VINČA" Institute of Nuclear Sciences - National Institute of the Republic of Serbia, Center of Excellence for Photoconversion, PO Box 522, Belgrade 11001, Serbia; Department of Experimental and Health Sciences, Pompeu Fabra University, Carrer del Dr. Aiguader 88, Barcelona 08003, Spain.
Colloids Surf B Biointerfaces. 2023 Jul;227:113340. doi: 10.1016/j.colsurfb.2023.113340. Epub 2023 May 12.
Gold nanoparticles were functionalized by amino acid tryptophan and vitamin riboflavin - a resonance energy transfer (RET) pair of biomolecules. The presence of the gold nanoparticles resulted in 65% increase in RET efficiency. Because of enhanced RET efficiency, the photobleaching dynamics of the fluorescent molecules at the surface of the nanoparticles is different from that of molecules in solution. The observed effect was used for detection of the functionalized nanoparticles within biological material rich with autofluorescent species. Synchrotron radiation deep-ultraviolet fluorescence microscopy is used to study the photobleaching dynamics of the fluorescence centers within human hepatocellular carcinoma Huh7.5.1 cells incubated with the nanoparticles. The fluorescent centers were classified according to their photobleaching dynamics, which enabled the discrimination of the cell areas where the accumulation of the nanoparticles takes place, even though the particles were smaller than the spatial resolution of the images.
金纳米粒子通过氨基酸色氨酸和维生素核黄素进行功能化——这是一对生物分子的共振能量转移 (RET) 对。金纳米粒子的存在使 RET 效率提高了 65%。由于增强的 RET 效率，纳米粒子表面荧光分子的光漂白动力学与溶液中分子的光漂白动力学不同。观察到的效果用于检测富含自体荧光物质的生物材料中的功能化纳米粒子。同步辐射深紫外荧光显微镜用于研究在与人肝癌 Huh7.5.1 细胞共孵育的金纳米粒子内的荧光中心的光漂白动力学。根据其光漂白动力学对荧光中心进行分类，即使颗粒小于图像的空间分辨率，也能够区分发生纳米颗粒积累的细胞区域。
