Adamska Elżbieta, Kowalska Agata, Wcisło Anna, Zima Katarzyna, Grobelna Beata
Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
Department of Physiology, Faculty of Medicine, Medical University of Gdansk, Debinki 1, 80-210 Gdansk, Poland.
Molecules. 2024 Dec 11;29(24):5837. doi: 10.3390/molecules29245837.
Gold nanoparticles (Au NPs) are a promising target for research due to their small size and the resulting plasmonic properties, which depend, among other things, on the chosen reducer. This is important because removing excess substrate from the reaction mixture is problematic. However, Au NPs are an excellent component of various materials, enriching them with their unique features. One example is hydrogels, which provide a good, easily modifiable base for multiple applications such as cosmetics. For this purpose, various compounds, including hyaluronic acid (HA) and its derivatives, are distinguished by their high water-binding capacity and many characteristics resulting from their natural origin in organisms, including biocompatibility, biodegradability, and tissue regeneration. In this work Au NPs were synthesized using a green chemistry method, either by using onion extract as a reductant or chemically reducing them with sodium citrate. A complete characterization of the nanoparticles was carried out using the following methods: Fourier-Transform Infrared Spectroscopy (FT-IR), Electrophoretic (ELS), and Dynamic Light Scattering (DLS) as well as Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Their antioxidant activity was also tested using the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that the synthesized nanoparticles enrich the hydrogels with antioxidant properties and new surface properties (depending on the reducing agent, they can be more hydrophilic or hydrophobic). Preliminary observations indicated low cytotoxicity of the nanomaterials in both liquid form and as a hydrogel component, as well as their lack of penetration through pig skin. The cosmetic properties of hydrogel masks were also confirmed, such as increasing skin hydration.
金纳米颗粒（Au NPs）因其尺寸小以及由此产生的等离子体特性而成为一个很有前景的研究目标，这些特性尤其取决于所选的还原剂。这一点很重要，因为从反应混合物中去除过量的底物存在问题。然而，金纳米颗粒是各种材料的优良成分，为它们赋予了独特的特性。一个例子是水凝胶，它为化妆品等多种应用提供了良好的、易于改性的基质。为此，包括透明质酸（HA）及其衍生物在内的各种化合物，因其高水结合能力以及源于生物体天然来源的许多特性而备受关注，这些特性包括生物相容性、生物降解性和组织再生能力。在这项工作中，金纳米颗粒采用绿色化学方法合成，要么使用洋葱提取物作为还原剂，要么用柠檬酸钠进行化学还原。使用以下方法对纳米颗粒进行了全面表征：傅里叶变换红外光谱（FT-IR）、电泳（ELS）、动态光散射（DLS）以及透射电子显微镜（TEM）和扫描电子显微镜（SEM）。还使用2,2-二苯基-1-苦基肼自由基（DPPH）测试了它们的抗氧化活性。结果表明，合成的纳米颗粒赋予水凝胶抗氧化性能和新的表面性能（取决于还原剂，它们可以更亲水或更疏水）。初步观察表明，纳米材料无论是液态还是作为水凝胶成分，细胞毒性都很低，并且它们不会穿透猪皮。水凝胶面膜的美容特性也得到了证实，比如增加皮肤水分含量。
