INTRODUCTION

Salmonellosis, which is typically distinguished by an immediate onset of fever, abdominal pain, diarrhea, nausea, and vomiting, is a bacterial infection caused by Salmonella. The rising incidence of antibiotic resistance in Typhimurium is a major worldwide problem, and a better knowledge of the distribution of antibiotic resistance patterns in is critical for selecting the best antibiotic for infection treatment. In this work, the efficiency of bacteriophage therapy of vegetative cells and biofilms of was investigated.

METHODS

Based on their host ranges, five Bacteriophages were chosen for therapy against 22 Salmonella isolates collected from various sources. PSCs1, PSDs1, PSCs2, PSSr1, and PSMc1 phages were found to exhibit potent anti- properties. In a 96-well microplate, the efficacy of bacteriophage therapy (10-10 PFU/mL) against biofilm formers was first tested. A bacteriophage treatment (10 PFU/mL) was subsequently applied in the laboratory for 24 hours to minimize adhering to the surfaces of gallstones and teeth. In 96-well microplate experiments, bacteriophage treatment inhibited biofilm development and reduced biofilm by up to 63.6% ( ≤ 0.05).

RESULT

When compared to controls, bacteriophages (PSCs1, PSDs1, PSCs2, PSSr1, PSMc1) demonstrated a rapid drop in the populations of biofilms generated on the surfaces of gallstones and teeth where the structure of the bacteria in the biofilm was broken and holes were created.

CONCLUSION

Clearly, this study indicated that phages might be employed to eliminate biofilms on gallstone and tooth surfaces.