Faculdade de Farmácia, Laboratório de Bioprospecção em Moléculas Antimicrobianas (LABIMAN), Universidade Federal do Ceará, Fortaleza, CE, CEP: 60.430-372, Brasil.
Centro de Pesquisa e Desenvolvimento de Fármacos (NPDM), Universidade Federal do Ceará, Fortaleza, CE, CEP: 60.430-275, Brasil.
Future Microbiol. 2023 May;18:415-426. doi: 10.2217/fmb-2022-0232. Epub 2023 May 22.
To evaluate the antibacterial activity of paroxetine alone and associated with oxacillin against isolates of methicillin-sensitive and -resistant . The broth microdilution and checkerboard techniques were used, with investigation of possible mechanisms of action through flow cytometry, fluorescence microscopy and molecular docking, in addition to scanning electron microscopy for morphological analysis. Paroxetine showed a MIC of 64 μg/ml and bactericidal activity, mostly additive interactions in combination with oxacillin, evidence of action on genetic material and membrane, morphological changes in microbial cells and influence on virulence factors. Paroxetine has antibacterial potential from the perspective of drug repositioning.
评估单独使用帕罗西汀和与苯唑西林联合使用对耐甲氧西林和甲氧西林敏感的 的抗菌活性。采用肉汤微量稀释和棋盘技术，并通过流式细胞术、荧光显微镜和分子对接研究可能的作用机制，此外还通过扫描电子显微镜进行形态分析。帕罗西汀的 MIC 为 64μg/ml，具有杀菌活性，与苯唑西林联合使用时主要表现为相加作用，作用于遗传物质和膜，微生物细胞形态发生变化，并影响毒力因子，提示帕罗西汀具有药物重定位的抗菌潜力。
