转录因子Ste12样蛋白增强菌丝体对非生物胁迫的耐受性并调节……的子实体发育。（原文此处“. ”表述不完整，可能有信息缺失）

The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development of .

作者信息

Lyu Xiaomeng, Wang Qingji, Liu Ao, Liu Fang, Meng Li, Wang Panmeng, Zhang Yan, Wang Li, Li Zhuang, Wang Wei

机构信息

Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an, China.

Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

出版信息

Front Microbiol. 2023 May 4;14:1139679. doi: 10.3389/fmicb.2023.1139679. eCollection 2023.

INTRODUCTION

is one of the most commercially important edible fungi worldwide, with its nutritional value and medicinal properties. It becomes a good model species to study the tolerance of abiotic stress during mycelia growth in edible mushroom cultivation. Transcription factor Ste12 has been reported to be involved in the regulation of stress tolerance and sexual reproduction in fungi.

METHODS

In this study, identification and phylogenetic analysis of was performed by bioinformatics methods. Four overexpression transformants of were constructed by Agrobacterium -mediated transformation.

RESULTS AND DISCUSSION

Phylogenetic analysis showed that Ste12-like contained conserved amino acid sequences. All the overexpression transformants were more tolerant to salt stress, cold stress and oxidative stress than wild-type strains. In the fruiting experiment, the number of fruiting bodies of overexpression transformants increased compared with wild-type strains, but the growth rate of stipes slowed down. It suggested that gene was involved in the regulation of abiotic stress tolerance and fruiting body development in .