Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning 110122, P. R. China.
Liaoning Key Laboratory of molecular targeted anti-tumor drug development and evaluation, Liaoning Cancer immune peptide drug Engineering Technology Research Center, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, Liaoning 110122, P. R. China.
Theranostics. 2023 Apr 23;13(8):2616-2631. doi: 10.7150/thno.83920. eCollection 2023.
Alternative splicing (AS) is a common and conserved process in eukaryotic gene regulation. It occurs in approximately 95% of multi-exon genes, greatly enriching the complexity and diversity of mRNAs and proteins. Recent studies have found that in addition to coding RNAs, non-coding RNAs (ncRNAs) are also inextricably linked with AS. Multiple different types of ncRNAs are generated by AS of precursor long non-coding (pre-lncRNAs) or precursor messenger RNAs (pre-mRNAs). Furthermore, ncRNAs, as a novel class of regulators, can participate in AS regulation by interacting with the cis-acting elements or trans-acting factors. Several studies have implicated abnormal expression of ncRNAs and ncRNA-related AS events in the initiation, progression, and therapy resistance in various types of cancers. Therefore, owing to their roles in mediating drug resistance, ncRNAs, AS-related factors and AS-related novel antigens may serve as promising therapeutic targets in cancer treatment. In this review, we summarize the interaction between ncRNAs and AS processes, emphasizing their great influences on cancer, especially on chemoresistance, and highlighting their potential values in clinical treatment.
可变剪接（AS）是真核基因调控中一种常见且保守的过程。它发生在大约 95%的多外显子基因中，极大地丰富了 mRNA 和蛋白质的复杂性和多样性。最近的研究发现，除了编码 RNA 外，非编码 RNA（ncRNA）也与 AS 密不可分。多种不同类型的 ncRNA 是通过前体长非编码 RNA（pre-lncRNA）或前体信使 RNA（pre-mRNA）的 AS 产生的。此外，ncRNA 作为一种新型的调控因子，可以通过与顺式作用元件或反式作用因子相互作用来参与 AS 调控。一些研究表明，ncRNA 的异常表达和与 ncRNA 相关的 AS 事件与各种类型癌症的发生、进展和治疗耐药性有关。因此，由于它们在介导耐药性方面的作用，ncRNA、AS 相关因子和 AS 相关新型抗原可能成为癌症治疗中有前途的治疗靶点。在这篇综述中，我们总结了 ncRNA 与 AS 过程之间的相互作用，强调了它们对癌症，特别是化疗耐药性的巨大影响，并强调了它们在临床治疗中的潜在价值。
