Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610061, China.
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, U.S.A.
Plant Dis. 2023 Nov;107(11):3585-3591. doi: 10.1094/PDIS-11-22-2600-RE. Epub 2023 Nov 6.
Wheat stripe rust, caused by f. sp. , is one of the most serious plant diseases worldwide. Resistant cultivars are the most effective way to control the disease. is an important stripe rust resistance gene that has been used in wheat breeding programs and is represented in the host differential set to identify f. sp. races in the United States. To map , AvSYrTr1NIL was backcrossed to its recurrent parent Avocet S (AvS). Seedlings of BCF, BCF, and BCF populations were tested with -avirulent races under controlled conditions, and BCF plants were genotyped using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. was mapped to the short arm of chromosome 1B using four SSR and seven SNP markers. The genetic distances of from the nearest flanking markers and were 1.8 and 1.3 centimorgans (cM), respectively. DNA amplification of a set of 21 Chinese Spring (CS) nulli-tetrasomic lines and seven CS 1B deletion lines with three SSR markers confirmed the chromosome arm location and further placed the gene in chromosomal bin region 1BS18 (0.5). The gene was determined to be about 7.4 cM proximal to . Based on multirace response array and chromosomal location, was determined to be different from other permanently named stripe rust resistance genes in chromosome arm 1BS and was named .
小麦条锈病由 f. sp. 引起，是全球最严重的植物病害之一。抗性品种是控制该病害最有效的方法。 是一个重要的条锈病抗性基因，已被用于小麦育种计划，并在美国的宿主差异组中用于鉴定 f. sp. 品种。为了定位 ，AvSYrTr1NIL 被回交到其轮回亲本 Avocet S（AvS）。在受控条件下，用 -毒力菌株测试 BCF、BCF 和 BCF 群体的幼苗，并用简单重复序列（SSR）和单核苷酸多态性（SNP）标记对 BCF 植株进行基因型分析。利用四个 SSR 和七个 SNP 标记将 定位到 1B 染色体的短臂上。 与最近的侧翼标记 和 之间的遗传距离分别为 1.8 和 1.3 厘摩（cM）。用一组 21 个中国春（CS）缺四体系和七个 CS 1B 缺失系的三个 SSR 标记进行 DNA 扩增，证实了染色体臂的位置，并将基因进一步定位在染色体 bin 区 1BS18（0.5）。基因被确定约在 7.4 cM 近端。基于多菌系反应阵列和染色体位置， 被确定与 1BS 染色体臂上的其他永久性命名的条锈病抗性基因不同，并被命名为 。
