Wang Xianyun, Hu Shiqi, Zhu Dashuai, Li Junlang, Cheng Ke, Liu Gang
Department of Cardiology, The First Hospital of Hebei Medical University, Shijiazhuang, 050000 China.
Scientific Research Data Center, The First Hospital of Hebei Medical University, Shijiazhuang, 050000 China.
Nano Res. 2023;16(5):7248-7259. doi: 10.1007/s12274-023-5374-3. Epub 2023 Feb 28.
Extracellular vesicles (EVs) generated from mesenchymal stem cells (MSCs) play an essential role in modulating cell-cell communication and tissue regeneration. The clinical translation of EVs is constrained by the poor yield of EVs. Extrusion has recently become an effective technique for producing a large scale of nanovesicles (NVs). In this study, we systematically compared MSC NVs (from extrusion) and EVs (from natural secretion). Proteomics and RNA sequencing data revealed that NVs resemble MSCs more closely than EVs. Additionally, microRNAs in NVs are related to cardiac repair, fibrosis repression, and angiogenesis. Lastly, intravenous delivery of MSC NVs improved heart repair and cardiac function in a mouse model of myocardial infarction.
Supplementary material (Figs. S1-S4) is available in the online version of this article at 10.1007/s12274-023-5374-3.
间充质干细胞（MSC）产生的细胞外囊泡（EV）在调节细胞间通讯和组织再生中起重要作用。EV的临床转化受到其产量低的限制。挤压法最近已成为一种大规模生产纳米囊泡（NV）的有效技术。在本研究中，我们系统地比较了MSC NV（来自挤压法）和EV（来自自然分泌）。蛋白质组学和RNA测序数据显示，NV比EV更类似于MSC。此外，NV中的微小RNA与心脏修复、纤维化抑制和血管生成有关。最后，在心肌梗死小鼠模型中，静脉注射MSC NV可改善心脏修复和心脏功能。
补充材料（图S1 - S4）可在本文的在线版本中获取，链接为10.1007/s12274-023-5374-3。
