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用于生物技术探索对……抗性的载体的阶段性葡萄基因组序列。 你提供的原文似乎不太完整，“resistance to”后面缺少具体内容。

Phased grapevine genome sequence of an carrier for biotechnological exploration of resistance to .

作者信息

Frommer Bianca, Müllner Sophia, Holtgräwe Daniela, Viehöver Prisca, Huettel Bruno, Töpfer Reinhard, Weisshaar Bernd, Zyprian Eva

机构信息

Genetics and Genomics of Plants, Faculty of Biology and Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.

Computational Biology, Faculty of Biology and Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.

出版信息

Front Plant Sci. 2023 May 8;14:1180982. doi: 10.3389/fpls.2023.1180982. eCollection 2023.

DOI:10.3389/fpls.2023.1180982
PMID:37223784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10200900/
Abstract

The downy mildew disease caused by the oomycete is a serious threat for grapevine and can cause enormous yield losses in viticulture. The quantitative trait locus , mediating resistance against , was originally found in Asian . This locus and its genes were analyzed here in detail. A haplotype-separated genome sequence of the diploid -carrier Gf.99-03 was created and annotated. The defense response against was investigated in an infection time-course RNA-seq experiment, revealing approximately 600 upregulated genes during host-pathogen interaction. The regions of the resistance and the sensitivity encoding Gf.99-03 haplotype were structurally and functionally compared with each other. Two different clusters of resistance-related genes were identified within the locus. One cluster carries a set of four differentially expressed genes with three 6-like genes. The other cluster carries a set of six resistance gene analogs related to qualitative pathogen resistance. The locus and its candidate genes for resistance provide a precious genetic resource for resistance breeding. Newly developed co-segregating simple sequence repeat markers in close proximity to the -genes enable its improved applicability in marker-assisted grapevine breeding.

摘要

由卵菌引起的霜霉病对葡萄构成严重威胁，会给葡萄栽培带来巨大产量损失。介导对霜霉病抗性的数量性状位点最初在亚洲葡萄中发现。本文对该位点及其基因进行了详细分析。构建并注释了二倍体霜霉病抗性载体Gf.99 - 03的单倍型分离基因组序列。在感染时间进程RNA测序实验中研究了对霜霉病的防御反应，结果显示在宿主 - 病原体相互作用期间约有600个基因上调。对Gf.99 - 03单倍型的抗性和敏感编码区域进行了结构和功能上的比较。在该数量性状位点内鉴定出两个不同的抗性相关基因簇。一个簇包含一组四个差异表达基因，其中有三个类似NBS-LRR的基因。另一个簇包含一组六个与定性病原体抗性相关的抗性基因类似物。该数量性状位点及其对霜霉病抗性的候选基因为霜霉病抗性育种提供了宝贵的遗传资源。新开发的与霜霉病抗性基因紧密连锁的共分离简单序列重复标记提高了其在标记辅助葡萄育种中的适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/02fc80addf25/fpls-14-1180982-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/dfb28053afb4/fpls-14-1180982-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/50c20ba385a1/fpls-14-1180982-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/931680e90f07/fpls-14-1180982-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/45da7b7b4a8e/fpls-14-1180982-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/02fc80addf25/fpls-14-1180982-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/dfb28053afb4/fpls-14-1180982-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/50c20ba385a1/fpls-14-1180982-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/931680e90f07/fpls-14-1180982-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/45da7b7b4a8e/fpls-14-1180982-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d315/10200900/02fc80addf25/fpls-14-1180982-g005.jpg

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