Possamai Tyrone, Migliaro Daniele, Gardiman Massimo, Velasco Riccardo, De Nardi Barbara
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, via delle Scienze 206, 33100 Udine, Italy.
CREA - Research Centre for Viticulture and Enology, viale XXVIII Aprile 26, 31015 Conegliano (TV), Italy.
Plants (Basel). 2020 Jun 22;9(6):781. doi: 10.3390/plants9060781.
Downy mildew, caused by the biotrophic oomycete , is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-assisted-selection (MAS) provide fast and cost-effective genotyping methods, but phenotyping remains necessary to characterize the host-pathogen interaction and assess the effective resistance level of new varieties as well as to validate MAS selection. In this study, the mediated defense responses were investigated in 31 genotypes, encompassing susceptible and resistant varieties and 26 seedlings, following inoculation of leaf discs with . The offspring differed in loci inherited (none, one or two): and from Solaris and and from Kozma 20-3. To improve the assessment of different resistance responses, pathogen reaction (sporulation) and host reaction (necrosis) were scored separately as independent features. They were differently expressed depending on locus: offspring carrying and loci showed the strongest resistance response (scarce sporulation and necrosis), those carrying locus showed the highest levels of necrosis while carrying genotypes showed intermediate levels of both sporulation and necrosis.
霜霉病由活体营养型卵菌引起，是葡萄最严重的病害之一。通过传统育种培育出对霜霉病具有部分抗性的新品种，为病害管理提供了一种可持续且有效的解决方案。标记辅助选择（MAS）提供了快速且经济高效的基因分型方法，但仍需要进行表型分析，以表征宿主 - 病原体相互作用、评估新品种的有效抗性水平以及验证MAS选择。在本研究中，在用[具体病原菌名称未给出]接种叶片圆盘后，对31个基因型（包括易感品种和抗性品种以及26株幼苗）中的介导防御反应进行了研究。后代在遗传的[具体基因座名称未给出]位点上存在差异（无、一个或两个）：来自Solaris的[具体基因座名称未给出]和[具体基因座名称未给出]，以及来自Kozma 20 - 3的[具体基因座名称未给出]和[具体基因座名称未给出]。为了改进对不同抗性反应的评估，将病原体反应（产孢）和宿主反应（坏死）作为独立特征分别评分。它们根据[具体基因座名称未给出]位点的不同而有不同表达：携带[具体基因座名称未给出]和[具体基因座名称未给出]位点的后代表现出最强的抗性反应（产孢和坏死较少），携带[具体基因座名称未给出]位点的后代坏死水平最高，而携带[具体基因座名称未给出]基因型的后代产孢和坏死水平处于中间。
