State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Infection Management Office, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China.
Int J Syst Evol Microbiol. 2023 May;73(5). doi: 10.1099/ijsem.0.005875.
A polyphasic taxonomic characterization of two novel strain pairs (designated zg-579/zg-578 and zg-536/zg-ZUI104) isolated from the faeces of was conducted based on phylogenetic analysis of the nearly full-length 16S rRNA gene and genome, digital DNA-DNA hybridization, ortho-average nucleotide identity (Ortho-ANI), and phenotypic and chemotaxonomic traits. Comparative analysis of the nearly full-length 16S rRNA gene sequences showed that strain zg-579 was most closely related to FR1436 (97.57 %) and SC8A-24 (97.36 %), whereas strain zg-536 had the highest similarity to MN8 (98.33 %), W2-2-3 (98.26 %) and 2C1-5 (98.19 %). Low levels of DNA-DNA relatedness and Ortho-ANI values (19.8-31.0 %/78.6-88.2 %, zg-579; 19.9-31.3 %/78.8-86.2 %, zg-536) between the two new type strains and previously known species within the genus support the hypothesis that the four newly characterized strains could be considered to represent two novel species within this genus. The dominant cellular fatty acids found in strain pair zg-536/zg-ZUI104 were iso-C and C 9, whereas C 8 was major component in zg-579/zg-578. Galactose and ribose were the main cell-wall sugars in these two new strain pairs. Diphosphatidylglycerol (DPG), phosphatidylcholine, phosphatidylglycerol (PG) and phosphatidylinositol (PI) were the major polar lipids in zg-579, whereas DPG, PG and PI predominated in zg-536. Both strain pairs had MK8(H) as the major respiratory quinone and ll-diaminopimelic acid as the major cell-wall peptidoglycan. The optimal growth conditions for the two novel strain pairs were 30 °C, pH 7.0 and 0.5 % NaCl (w/v). Based on these polyphasic characterizations, two novel species within the genus are proposed, i.e. sp. nov. and sp. nov., with zg-579 (=CGMCC 4.7663=JCM 33892) and zg-536 (=CGMCC 4.7662=JCM 33891) as the type strains.
基于近全长 16S rRNA 基因和基因组、数字 DNA-DNA 杂交、正交平均核苷酸同一性 (Ortho-ANI) 以及表型和化学分类特征，对两株新型菌株 (zg-579/zg-578 和 zg-536/zg-ZUI104) 进行了多相分类学特征描述，这些菌株均从粪便中分离得到。结果表明，菌株 zg-579 与 FR1436 (97.57%) 和 SC8A-24 (97.36%) 的近全长 16S rRNA 基因序列最为相似，而菌株 zg-536 与 MN8 (98.33%)、W2-2-3 (98.26%) 和 2C1-5 (98.19%) 的相似度最高。这两个新菌株与属内先前已知种之间的 DNA-DNA 同源性和 Ortho-ANI 值较低 (19.8-31.0%/78.6-88.2%，zg-579；19.9-31.3%/78.8-86.2%，zg-536)，支持这四个新鉴定的菌株可被认为是该属内两个新种的假设。菌株对 zg-536/zg-ZUI104 的主要细胞脂肪酸为 iso-C 和 C9，而 zg-579/zg-578 的主要成分是 C8。这两个新菌株对的主要细胞壁糖为半乳糖和核糖。这两个新菌株对的主要极性脂分别为二磷脂酰甘油 (DPG)、磷脂酰胆碱、磷脂酰甘油 (PG) 和磷脂酰肌醇 (PI)，而 DPG、PG 和 PI 则是 zg-536 的主要成分。两个菌株对均以 MK8(H) 作为主要呼吸醌，以 ll-二氨基庚二酸作为主要细胞壁肽聚糖。这两个新菌株对的最佳生长条件为 30°C、pH 7.0 和 0.5%NaCl (w/v)。基于这些多相特征，提出了属内两个新种，即 sp. nov.和 sp. nov.，其中 zg-579(=CGMCC 4.7663=JCM 33892)和 zg-536(=CGMCC 4.7662=JCM 33891)分别为它们的模式菌株。
