Odabas Sedat, Derkuş Burak, Vargel İbrahim, Vural Altughan Cahit
Ankara University, Faculty of Science, Department of Chemistry, Biomaterials and Tissue Engineering Laboratory (bteLAB), Turkey.
Interdisciplinary Research Unit for Advanced Materials (INTRAM), Ankara University, Ankara, Turkey.
MethodsX. 2023 May 4;10:102208. doi: 10.1016/j.mex.2023.102208. eCollection 2023.
Cranial tissue models are a widely used model to show the bone repair and the regeneration ability of candidate biomaterials for tissue engineering purposes. Until now, efficacy studies of different biomaterials for calvarial defect bone regeneration have been reported, generally in small animal models. This paper offers a versatile, reliable, and reproducible surgical method for creating a critical-sized cranial defect in rats including critical steps and tried-and-tested tips. The method proposed here,•Shows a general procedure for in vivo cranial models.•Provide an insight to restore bone tissue repair that may be used in combination with several tissue engineering strategies•Is a crucial technique that may guide in vivo bone tissue engineering.
颅骨组织模型是一种广泛应用的模型，用于展示骨修复以及用于组织工程目的的候选生物材料的再生能力。到目前为止，已有关于不同生物材料用于颅骨缺损骨再生的功效研究报道，这些研究一般是在小动物模型中进行的。本文提供了一种通用、可靠且可重复的手术方法，用于在大鼠中制造临界大小的颅骨缺损，包括关键步骤和经过实践检验的技巧。这里提出的方法，•展示了体内颅骨模型的一般程序。•为恢复骨组织修复提供了一种见解，可与多种组织工程策略联合使用•是一项关键技术，可能会指导体内骨组织工程。
