Pincemail Joël, Cavalier Etienne, Charlier Corinne, Cheramy-Bien Jean-Paul, Brevers Eric, Courtois Audrey, Fadeur Marjorie, Meziane Smail, Goff Caroline Le, Misset Benoît, Albert Adelin, Defraigne Jean-Olivier, Rousseau Anne-Françoise
Clinical Chemistry, CHU of Liège, Sart Tilman, 4000 Liège, Belgium.
Toxicology Department, CHU of Liège, Sart Tilman, 4000 Liège, Belgium.
Antioxidants (Basel). 2021 Feb 7;10(2):257. doi: 10.3390/antiox10020257.
A key role of oxidative stress has been highlighted in the pathogenesis of COVID-19. However, little has been said about oxidative stress status (OSS) of COVID-19 patients hospitalized in intensive care unit (ICU).
Biomarkers of the systemic OSS included antioxidants (9 assays), trace elements (3 assays), inflammation markers (4 assays) and oxidative damage to lipids (3 assays).
Blood samples were drawn after 9 (7-11) and 41 (39-43) days of ICU stay, respectively in 3 and 6 patients. Vitamin C, thiol proteins, reduced glutathione, γ-tocopherol, β-carotene and PAOT score were significantly decreased compared to laboratory reference values. Selenium concentration was at the limit of the lower reference value. By contrast, the copper/zinc ratio (as a source of oxidative stress) was higher than reference values in 55% of patients while copper was significantly correlated with lipid peroxides (r = 0.95, < 0.001). Inflammatory biomarkers (C-reactive protein and myeloperoxidase) were significantly increased when compared to normals.
The systemic OSS was strongly altered in critically ill COVID-19 patients as evidenced by increased lipid peroxidation but also by deficits in some antioxidants (vitamin C, glutathione, thiol proteins) and trace elements (selenium).
氧化应激在2019冠状病毒病（COVID-19）发病机制中的关键作用已得到强调。然而，对于入住重症监护病房（ICU）的COVID-19患者的氧化应激状态（OSS）却鲜有提及。
全身OSS的生物标志物包括抗氧化剂（9项检测）、微量元素（3项检测）、炎症标志物（4项检测）和脂质氧化损伤（3项检测）。
分别在3例和6例患者入住ICU的第9天（7 - 11天）和第41天（39 - 43天）采集血样。与实验室参考值相比，维生素C、硫醇蛋白、还原型谷胱甘肽、γ-生育酚、β-胡萝卜素和PAOT评分显著降低。硒浓度处于较低参考值的下限。相比之下，55%的患者铜/锌比值（作为氧化应激源）高于参考值，且铜与脂质过氧化物显著相关（r = 0.95，P < 0.001）。与正常人相比，炎症生物标志物（C反应蛋白和髓过氧化物酶）显著升高。
危重症COVID-19患者的全身OSS发生了显著改变，脂质过氧化增加以及某些抗氧化剂（维生素C、谷胱甘肽、硫醇蛋白）和微量元素（硒）缺乏均证明了这一点。
