Brennan Edwina, Butler Alexandra E, Nandakumar Manjula, Drage Daniel S, Sathyapalan Thozhukat, Atkin Stephen L
School of Medicine, Royal College of Surgeons in Ireland-Medical University of Bahrain, Busaiteen 15503, Bahrain.
School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Biomedicines. 2023 May 15;11(5):1451. doi: 10.3390/biomedicines11051451.
In human population studies, organochlorine pesticides (OCPs) have been linked to vitamin D deficiency. Therefore, this study examined the association between OCPs, vitamin D (cholecalciferol, 25(OH)D), and the active metabolite 1,25-dihydrovitamin D (1,25(OH)D) in a cohort of non-obese women. The serum samples of 58 female participants (age-31.9 ± 4.6 years; body mass index (BMI)-25.7 ± 3.7 kg/m) were screened for 10 indicator OCPs. 25(OH)D and 1,25(OH)D levels were determined via isotope dilution liquid chromatography tandem mass spectrometry. In this cohort, the 25(OH)D and 1,25(OH)D levels were 22.9 ± 11.2 ng/mL and 0.05 ± 0.02 ng/mL, respectively, with 28 participants classified as 25(OH)D-deficient (<50 nmol/L). In the study cohort, no correlations were found between individual or total OCPs (ƩOCPs) and 25(OH)D. p,p'-dichlorodiphenyldichloroethylene (DDE) and ƩOCPs correlated positively with 1,25(OH)D, with the latter being negatively correlated with estimated glomerular filtration rate (eGFR). In women with sufficient 25(OH)D levels, p,p'-dichlorodiphenyltrichloroethan (DDT) was positively correlated with 1,25(OH)D, whilst in the deficient group, hexachlorobenzene (HCB) and p,p'-(DDE) were positively correlated with 1,25(OH)D, β-Hexachlorocyclohexane (HCH) was positively correlated with 25(OH)D, and none of the OCPs were associated with measures of renal function. Overall, OCPs and ƩOCPs were not associated with 25(OH)D, suggesting that they are unrelated to vitamin D deficiency, but p,p'-DDE and ƩOCPs correlated positively with active 1,25(OH)D, while ƩOCPs correlated negatively with eGFR, suggesting a possible renal effect. Analysis of vitamin D deficiency revealed an association between β-HCH and 25(OH)D, and between HCB and p,p'-DDE and 1,25(OH)D, suggesting that OCP effects may be enhanced in cases of vitamin D deficiency.
在人群研究中，有机氯农药（OCPs）与维生素D缺乏有关。因此，本研究在一组非肥胖女性队列中，考察了OCPs、维生素D（胆钙化醇、25(OH)D）和活性代谢物1,25 - 二羟维生素D（1,25(OH)D）之间的关联。对58名女性参与者（年龄31.9±4.6岁；体重指数（BMI）25.7±3.7kg/m²）的血清样本进行了10种指示性OCPs筛查。通过同位素稀释液相色谱串联质谱法测定25(OH)D和1,25(OH)D水平。在该队列中，25(OH)D和1,25(OH)D水平分别为22.9±11.2ng/mL和0.05±0.02ng/mL，28名参与者被归类为25(OH)D缺乏（<50nmol/L）。在研究队列中，未发现单个或总OCPs（ƩOCPs）与25(OH)D之间存在相关性。p,p'-二氯二苯二氯乙烯（DDE）和ƩOCPs与1,25(OH)D呈正相关，后者与估计肾小球滤过率（eGFR）呈负相关。在25(OH)D水平充足的女性中，p,p'-二氯二苯三氯乙烷（DDT）与1,25(OH)D呈正相关，而在缺乏组中，六氯苯（HCB）和p,p'-(DDE)与1,25(OH)D呈正相关，β-六氯环己烷（HCH）与25(OH)D呈正相关，且没有OCPs与肾功能指标相关。总体而言，OCPs和ƩOCPs与25(OH)D无关，表明它们与维生素D缺乏无关，但p,p'-DDE和ƩOCPs与活性1,25(OH)D呈正相关，而ƩOCPs与eGFR呈负相关，提示可能存在肾脏效应。维生素D缺乏分析显示，β-HCH与25(OH)D之间、HCB和p,p'-DDE与1,25(OH)D之间存在关联，表明在维生素D缺乏的情况下，OCPs的影响可能会增强。
