College of Life Sciences, Shandong Normal University, Jinan 250014, China.
School of Biosciences, University of Nottingham Malaysia, Semenyih 43500, Malaysia.
Int J Mol Sci. 2023 May 11;24(10):8623. doi: 10.3390/ijms24108623.
Genome editing is an important strategy to maintain global food security and achieve sustainable agricultural development. Among all genome editing tools, CRISPR-Cas is currently the most prevalent and offers the most promise. In this review, we summarize the development of CRISPR-Cas systems, outline their classification and distinctive features, delineate their natural mechanisms in plant genome editing and exemplify the applications in plant research. Both classical and recently discovered CRISPR-Cas systems are included, detailing the class, type, structures and functions of each. We conclude by highlighting the challenges that come with CRISPR-Cas and offer suggestions on how to tackle them. We believe the gene editing toolbox will be greatly enriched, providing new avenues for a more efficient and precise breeding of climate-resilient crops.
基因组编辑是维护全球粮食安全和实现可持续农业发展的重要策略。在所有的基因组编辑工具中，CRISPR-Cas 目前最为流行，也最有前景。在这篇综述中，我们总结了 CRISPR-Cas 系统的发展，概述了它们的分类和特点，阐述了它们在植物基因组编辑中的自然机制，并举例说明了它们在植物研究中的应用。我们涵盖了经典和最近发现的 CRISPR-Cas 系统，详细介绍了每一种系统的类别、类型、结构和功能。最后，我们强调了 CRISPR-Cas 带来的挑战，并就如何应对这些挑战提出了建议。我们相信，基因编辑工具将大大丰富，为培育更具抗逆性的作物提供更高效、更精确的新途径。
