Tecnologico de Monterrey, School of Engineering and Sciences, Campus Querétaro, Av. Epigmenio González, No. 500 Fracc. San Pablo, 76130, Querétaro, Mexico.
Tecnologico de Monterrey, School of Engineering and Sciences, Campus Puebla, Atlixcáyotl 5718, Reserva Territorial Atlixcáyotl, 72453, Puebla, Mexico.
Planta. 2023 Dec 28;259(2):32. doi: 10.1007/s00425-023-04303-z.
CRISPR/Cas technology has greatly facilitated plant non-coding RNA (ncRNA) biology research, establishing itself as a promising tool for ncRNA functional characterization and ncRNA-mediated plant improvement. Throughout the last decade, the promising genome editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas; CRISPR/Cas) has allowed unprecedented advances in the field of plant functional genomics and crop improvement. Even though CRISPR/Cas-mediated genome editing system has been widely used to elucidate the biological significance of a number of plant protein-coding genes, this technology has been barely applied in the functional analysis of those non-coding RNAs (ncRNAs) that modulate gene expression, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). Nevertheless, compelling findings indicate that CRISPR/Cas-based ncRNA editing has remarkable potential for deciphering the biological roles of ncRNAs in plants, as well as for plant breeding. For instance, it has been demonstrated that CRISPR/Cas tool could overcome the challenges associated with other approaches employed in functional genomic studies (e.g., incomplete knockdown and off-target activity). Thus, in this review article, we discuss the current status and progress of CRISPR/Cas-mediated ncRNA editing in plant science in order to provide novel prospects for further assessment and validation of the biological activities of plant ncRNAs and to enhance the development of ncRNA-centered protocols for crop improvement.
CRISPR/Cas 技术极大地促进了植物非编码 RNA（ncRNA）生物学研究，成为研究 ncRNA 功能特征和 ncRNA 介导的植物改良的有前途的工具。在过去的十年中，有前途的基因组编辑工具簇状规则间隔短回文重复（CRISPR）/CRISPR 相关蛋白（Cas；CRISPR/Cas）允许在植物功能基因组学和作物改良领域取得前所未有的进展。尽管 CRISPR/Cas 介导的基因组编辑系统已广泛用于阐明许多植物蛋白编码基因的生物学意义，但该技术几乎没有应用于调节基因表达的非编码 RNA（ncRNA）的功能分析，例如 microRNAs（miRNAs）和长非编码 RNA（lncRNAs）。然而，令人信服的发现表明，基于 CRISPR/Cas 的 ncRNA 编辑在破译 ncRNA 在植物中的生物学作用以及植物育种方面具有巨大的潜力。例如，已经证明 CRISPR/Cas 工具可以克服功能基因组研究中使用的其他方法（例如不完全敲低和脱靶活性）所带来的挑战。因此，在这篇综述文章中，我们讨论了 CRISPR/Cas 介导的 ncRNA 编辑在植物科学中的现状和进展，以便为进一步评估和验证植物 ncRNA 的生物学活性提供新的前景，并增强以 ncRNA 为中心的作物改良方案的发展。
