Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630117, Russia.
Faculty of Natural Sciences, Novosibirsk State University, Novosibirsk 630073, Russia.
Viruses. 2023 May 7;15(5):1121. doi: 10.3390/v15051121.
Avian coronaviruses (ACoV) have been shown to be highly prevalent in wild bird populations. More work on avian coronavirus detection and diversity estimation is needed for the breeding territories of migrating birds, where the high diversity and high prevalence of and have already been shown in wild birds. In order to detect ACoV RNA, we conducted PCR diagnostics of cloacal swab samples from birds, which we monitored during avian influenza A virus surveillance activities. Samples from two distant Asian regions of Russia (Sakhalin region and Novosibirsk region) were tested. Amplified fragments of the RNA-dependent RNA-polymerase (RdRp) of positive samples were partially sequenced to determine the species of represented. The study revealed a high presence of ACoV among wild birds in Russia. Moreover, there was a high presence of birds co-infected with avian coronavirus, avian influenza virus, and avian paramyxovirus. We found one case of triple co-infection in a Northern Pintail (). Phylogenetic analysis revealed the circulation of a species. A species was not detected, which supports the data regarding the low prevalence of deltacoronaviruses among surveyed bird species.
禽冠状病毒（ACoV）在野生鸟类种群中高度流行。对于候鸟的繁殖地，需要更多关于禽冠状病毒检测和多样性估计的工作，因为已经在野生鸟类中发现了的高多样性和高流行率。为了检测 ACoV RNA，我们对在禽流感病毒监测活动中监测到的鸟类的泄殖腔拭子样本进行了 PCR 诊断。测试了来自俄罗斯两个遥远地区（萨哈林地区和新西伯利亚地区）的样本。对阳性样本的 RNA 依赖性 RNA 聚合酶（RdRp）的扩增片段进行了部分测序，以确定代表的。研究表明，俄罗斯野生鸟类中存在高浓度的 ACoV。此外，还存在大量同时感染禽冠状病毒、禽流感病毒和禽副粘病毒的鸟类。我们在一只北方针尾鸭（）中发现了一例三重感染。系统发育分析显示循环的种。未检测到种，这支持了关于在所调查的鸟类物种中德尔塔冠状病毒低流行率的数据。
