College of Animal Sciences and Technologies, Anhui Agricultural University, Hefei, 230036, China.
School of Medicine, Jiangsu University, 310 Xuefu Road, Zhenjiang, 202103, Jiangsu, China.
Arch Virol. 2020 Mar;165(3):703-707. doi: 10.1007/s00705-019-04488-3. Epub 2020 Jan 25.
Using viral metagenomics, the complete genome sequence of an infectious bronchitis virus (IBV) strain (named ahysx-1) from a fecal sample from a healthy chicken in Anhui province, China, was determined. The genome sequence of ahysx-1 was found to be very similar to that of IBV strain ck/CH/LLN/131040 (KX252787), except for the spike gene region, which is similar to that of a turkey coronavirus strain (EU022526), suggesting that ahysx-1 is a recombinant. Recombination analysis and phylogenetic analysis based on the genomic sequences of ahysx-1 and other related strains confirmed that ahysx-1 appears to be a recombinant resulting from a recombination event that occurred between a chicken coronavirus and a turkey coronavirus. Further studies need to be performed to determine whether this recombinant IBV strain is pathogenic and whether it is transmitted between chickens and turkeys.
利用病毒宏基因组学，从中国安徽省一只健康鸡的粪便样本中分离出一株传染性支气管炎病毒（IBV）株（命名为 ahysx-1），并确定了其全基因组序列。ahysx-1 的基因组序列与 IBV 株 ck/CH/LLN/131040（KX252787）非常相似，除了刺突基因区域，该区域与一株火鸡冠状病毒株（EU022526）相似，提示 ahysx-1 是一种重组病毒。基于 ahysx-1 和其他相关株的基因组序列进行的重组分析和系统进化分析证实，ahysx-1 似乎是一种重组病毒，是由鸡冠状病毒和火鸡冠状病毒之间的重组事件产生的。需要进一步研究来确定这种重组型 IBV 株是否具有致病性，以及它是否在鸡和火鸡之间传播。
